in this study, effect of exogenous putrescine on maintaining postharvest quality and antioxidant compounds of two broccoli cultivars, ‘general’ and ‘liberty’ during cool storage were investigated. broccoli branchlets were treated with 0.5, 1 and 1.5 mm putrescine. distilled water was used as control. treated florets were put into polyethylene bag and transferred to storage with 0°c and 90% rh. ...

The effectiveness of a series of sulfhydryl compounds in inhibiting polyphenoloxidase (PPO) activity in a model system (chlorogenic acid solution) and in loquat juices was evaluated. Application of different concentrations of sulfhydryl compounds to chlorogenic acid solution and fresh loquat juice showed that l-cysteine appeared to be an effective browning inhibitor. The required concentration ...

An extracellular chlorogenic acid esterase from Ustilago maydis (UmChlE) was purified to homogeneity by using three separation steps, including anion-exchange chromatography on a Q Sepharose FF column, preparative isoelectric focusing (IEF), and, finally, a combination of affinity chromatography and hydrophobic interaction chromatography on polyamide. SDS-PAGE analysis suggested a monomeric pro...

To investigate the enzyme variations responsible for the synthesis of phenolics, 40 day-old adventitious roots of Panax ginseng were treated with 200 microM methyl jasmonate (MJ) or salicylic acid (SA) in a 5 L bioreactor suspension culture (working volume 4 L). Both treatments caused an increase in the carbonyl and hydrogen peroxide (H2O2) contents, although the levels were lower in SA treated...

Peach blossoms were harvested and classified into six developmental stages: (I) bud emerging stage; (II) middle bud stage; (III) large bud stage; (IV) initial-flowering stage; (V) full-flowering stage; and (VI) end-flowering stage. The contents of total phenolics, flavanoids, individual phenolic compounds as well as antioxidant and tyrosinase inhibitory activity of peach blossoms at different d...

OBJECTIVE Coffee consumption has been associated with lower risk of type 2 diabetes. We evaluated the acute effects of decaffeinated coffee and the major coffee components chlorogenic acid and trigonelline on glucose tolerance. RESEARCH DESIGN AND METHODS We conducted a randomized crossover trial of the effects of 12 g decaffeinated coffee, 1 g chlorogenic acid, 500 mg trigonelline, and place...

The in vitro antibacterial and antibiofilm activity of chlorogenic acid against clinical isolates of Stenotrophomonas maltophilia was investigated through disk diffusion, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), time-kill and biofilm assays. A total of 9 clinical S. maltophilia isolates including one isolate resistant to trimethoprim/sulfamethoxazole (TM...

Extracts from ripe fruit and seeds of Sorbus aucuparia inhibit germination of other seeds, tested with Amaranthus caudatus and Lepidium sativum. The main compound of the “neutral fraction” of fruit extracts is the lactone compound parasorbic acid (4 —7 mg/g fresh weight) which inhibits germination at concentrations > 5x1C T 4 m. The “acid fraction” contains abscisic acid (1 .3—2.5 ^ / g fresh w...

The present study reveals the pharmacological effect of chlorogenic acid, a major phenolic compound in plants, food, and coffee, on diabetic cataracts. Chlorogenic acid examined the inhibitory effects upon rat lens aldose reductase (AR) activity and xylose-induced rat lens opacity. The effect of chlorogenic acid on high glucose-induced cytotoxicity in lens epithelial cells was also examined. Ch...

Electrospun nanofibers have gathered more consideration in worldwide to deliver the bioactive agents in a sustained manner for biomedical applications. In this study, chlorogenic acid loaded polycaprolactone nanofibers were fabricated by electrospinning process and the average fiber diameters were analyzed using scanning electron microscopy analysis. Further, Fourier transform infrared spectros...