MOTIVATION Pyrosequencing is a cost-effective DNA sequencing technology that has many applications, including rapid genotyping of a broad spectrum of bacteria. When molecular typing requires to genotype multiple DNA stretches, several pyrosequencing primers could be used simultaneously but this would create overlapping primer-specific signals, which are visually uninterpretable. Accordingly, th...

This work primarily investigated the pyrolysis of smalls scale fixed bed reactor using plastic wastes type LDPE during slow (non-isothermal) in a batch to assess effect different temperatures on product yield and heating value. The calorie bomb Parr Instrument was performed value pyro-oil at 250, 275, 300oC. obtained higher 300 oC while increased temperature will affect increase pyro-oil. highe...

Phosphorus (P) from biomass can cause operational problems in thermal conversion processes. In order to explore the release mechanism of P gas phase, carbothermic reduction meta-, pyro-, and orthophosphates ash elements commonly found biomass; sodium, potassium, magnesium, calcium was investigated. Mixtures each phosphate activated carbon were heated 1135 °C a laboratory-scale reactor, with CO ...

Fuel oil used in power plants contain metal impurities like Vanadium. After combustion, this metal remains in boiler fuel ash in the form of oxide. In this research, extraction of Vanadium oxide from fuel ash has been investigated. Two processes were used for this purpose. A pyro- hydrometallurgy and a hydrometallurgy process. In the pyro-hydrometallurgy process, using sodium carbonate, salt ro...

The gut microbiota is of crucial importance for the host with considerable metabolic activity. Although great efforts have been made toward characterizing microbial diversity, measuring components' metabolic activity surprisingly hasn't kept pace. Here we combined pyrosequencing of amplified 16S rRNA genes with in vivo stable isotope probing (Pyro-SIP) to unmask metabolically active bacteria in...

The development of activatable photodynamic therapy (PDT) has demonstrated a utility for effective photosensitizer quenchers. However, little is known quantitatively about Forster resonance energy transfer (FRET) quenching of photosensitizers, even though these quenchers are versatile and readily available. To characterize FRET deactivation of singlet oxygen generation, we attached various quen...