SUMMARY AS-30D and Novikoff rat ascites hepatoma cells were incubated in a medium selected according to the following criteria: (a) low leakage of lactate dehydrogenase; (b) low uptake of trypan blue; (c) high adenosine phosphate content; (‘0 high adenosine triphosphate/adenosine diphosphate ratio and adenylate energy charge. A procedure for defined cooling, freezing, and deproteinization of ...

A novel experimental pHand time-dependent colonic drug delivery system was prepared and its in vitro and in vivo performances were evaluated. The colonic delivery system consists of minitablets with a big swelling capacity coated with an inlet retardant layer insoluble at all pH ranges but permeable (Eudragit RS 30D) and an outlet layer, insoluble and impermeable at acidic pH but easily soluble...

Hexokinase type II is highly overexpressed in many cancer cells, where it plays a pivotal role in the high glycolytic phenotype. Here we demonstrate by Southern blot analysis and fluorescence in situ hybridization (FISH) that in the rapidly growing rat AS-30D hepatoma cell line, enhanced hexokinase activity is associated with at least a 5-fold amplification of the type II gene relative to norma...

Abstract Background MicroRNA (miR)-30d is not only a valuable biomarker for assessing the extent of cardiac remodeling after myocardial infarction (MI), but also an important predictor clinical outcome in heart failure. Overexpression miRNA-30d appears to have cardioprotective effect by preventing cardiomyocyte apoptosis as well averting fibroblast proliferation via multiple molecular pathways....

During embryo implantation, the blastocyst interacts with and regulates the endometrium, and endometrial fluid secreted by the endometrial epithelium nurtures the embryo. Here, we propose that maternal microRNAs (miRNAs) might act as transcriptomic modifier of the pre-implantation embryo. Microarray profiling revealed that six of 27 specific, maternal miRNAs were differentially expressed in the...

The Androgen Receptor (AR) plays a key role in prostate biology and in the progression of prostate cancer (PCa) to castration resistance. The role of microRNAs (miRNAs) in aberrant AR signaling have not been fully characterized. Here we screened a library of 810 miRNA mimics to identify miRNAs that alter AR activity in complementary functional assays including protein lysate microarray (LMA) qu...

Solid tumours have oxygen gradients and areas of near and almost total anoxia. Hypoxia reduces sensitivity to 5-fluorouracil (5-FU)-chemotherapy for colorectal cancer (CRC). MicroRNAs (miRNAs) are hypoxia sensors and were altered consistently in six CRC cell lines (colon cancer: DLD-1, HCT116 and HT29; rectal cancer: HT55, SW837 and VACO4S) maintained in hypoxia (1 and 0.2% oxygen) compared wit...

The aim of this study was to establish effect of 14 day consumption of commercially available yoghurt containing Lactobacillus rhamnosus ATCC53103 - LGG (Bioaktiv LGG, Dukat, Croatia) on Streptococcus mutans and Lactobacillus spp. salivary counts in children. Twenty five patients, 6-10 yr old participated in the study. At the inclusion in the study caries risk for every patient was evaluated. T...

Mitochondria of AS-30D rat ascites hepatoma cells are found to be the main target for Zn(2+) and sodium selenite (Na(2)SeO(3)). High [mu]M concentrations of Zn(2+) or selenite were strongly cytotoxic, killing the AS-30D cells by both apoptotic and necrotic ways. Both Zn(2+) and selenite produced strong changes in intracellular generation of reactive oxygen species (ROS) and the mitochondrial dy...

Aim: To determine any differences in visual acuity (VA) and contrast sensitivity (CS) between 3M Fresnel prisms and Trusetal prisms foils. Methods: Sixteen participants (mean age 20 years) with normal VA and CS participated. The effect of 5D, 10D, 20D and 30D prism strengths on monocular VA and CS was assessed using a Bailey-Lovie logMAR chart and Pelli-Robson chart, respectively. Measurements ...