Murine immune interferon (Mu-IFN-gamma) can be radiolabeled with [gamma-32P]ATP by the catalytic subunit of cAMP-dependent protein kinase. The resulting 32P-labeled Mu-IFN-gamma (32P-Mu-IFN-gamma) with high radiological specific activity (60-260 muCi/micrograms) retains biological activity. Acid hydrolysis of 32P-Mu-IFN-gamma or 32P-labeled human IFN-gamma leads to the release of [32P]phosphose...

Addition of the four deoxyribonucleosides in vitro to 6C3HED mouse ascites tumor stimulates 32P incorporation into DNA and depresses incorporation into RNA at concentrations greater than 10~4 M. The four ribonucleosides stimulate incorporation into both DNA and RNA. Hydroxyurea blocks the ribonucleoside stimulation of 32P incorporation into DNA but not RNA. The inhibition of 32P incorporation i...

Addition of the four deoxyribonucleosides in vitro to 6C3HED mouse ascites tumor stimulates 32P incorporation into DNA and depresses incorporation into RNA at concentrations greater than 10~4 M. The four ribonucleosides stimulate incorporation into both DNA and RNA. Hydroxyurea blocks the ribonucleoside stimulation of 32P incorporation into DNA but not RNA. The inhibition of 32P incorporation i...

Phosphorylation of the insulin-regulatable glucose transporter (IRGT) is increased by incubating rat adipocytes with isoproterenol or by incubating microsomal membranes with cAMP-dependent protein kinase. To attempt to locate the sites of phosphorylation, the IRGT (apparent Mr = 46,000) was immunoprecipitated from 32P-labeled adipocytes and cleaved with CNBr or trypsin. Essentially all of the 3...

Introduction Pterygium is a wing-shaped, vascular, fleshy growth that originates from the conjunctiva and can spread into the corneal limbus and beyond. Beta irradiation after bare sclera surgery of primary pterygium is a simple, effective, and safe treatment, which reduces the risk of local recurrence. Materials and Methods Dosimetric components of strontium-90 (90Sr), phosphorous-32 (32P), an...

Incorporation of 32P into yeast fructose-1,6-bisphosphatase (EC 3.1.3.11) was observed after addition of glucose to a cell suspension incubated with (32P)orthophosphoric acid. The 32P counts were coincident with the enzyme band when immunoprecipitates were subjected to sodium dodecyl sulfate disc gel electrophoresis. The incorporation of phosphate was associated with a decrease in enzyme activi...

The aim of the paper was to study the effect of carbisocaine, a new local anesthetic with high liposolubility on incorporation of 32P into individual and total phospholipids and to compare its effect with that of other local anesthetics (procaine, lidocaine, cinchocaine, heptacaine). Carbisocaine decreased 32P incorporation into neutral phospholipids and increased the incorporation into acid ph...

OBJECTIVE To evaluate whether nuclear activity as measured by the disodium phosphate 32P (32P) uptake test for uveal melanoma is of prognostic value and corresponds to known prognostic factors. METHODS A retrospective analysis of 121 patients with choroidal and/or ciliary body melanoma, tested with the 32P uptake test before enucleation between January 1, 1973, and December 31, 1976, at the L...