1. When rat stomach fundus muscle was incubated for 30 min in Tyrode solution from which calcium chloride had been omitted, there was an almost complete abolition of the contractile response to 5-hydroxytryptamine (5-HT) while that to acetylcholine (ACh) was still present. 2. The maximum tension obtainable with ACh remained the same in external calcium concentrations ranging from 0.45 to 3.6 mM...

Seven Bacillus spp. were exposed to simulations of Mars-normal UV fluence rates in order to study the effects of UV irradiation on microbial survival. A UV illumination system was calibrated to deliver 9.78 W m−2 (35.2 kJ m−2 h−1) of UVC + UVB irradiation (200–320 nm) to microbial samples, thus creating a clear-sky simulation (0.5 optical depth) of equatorial Mars. The Bacillus spp. studied wer...

Publicly available Digital Elevation Models (DEM) derived from various space-based platforms (Satellite/Space Shuttle Endeavour) have had a tremendous impact on the quantification of landscape characteristics, and related processes products. The accuracy elevation data six major public domain satellite-derived (a 30 m grid size—ASTER GDEM version 3 (Ast30), SRTM (Srt30), CartoDEM V3R1 (Crt30)—a...

A satisfactory LC-MS/MS separation and good peak symmetry for Atrasentan were obtained with Agilent, Zorbax, XDB C18 (2.1 x 50 mm ID, 5 ?m), a mobile phase containing mixture of mM Ammonium Formate buffer 0.1% formic acid mixed HPLC grade Acetonitrile in the proportion 70:30, v/v was delivered at flow rate 0.150 mL/min by positive ion mode (API 4000) an injection volume 10 µL run time 3 min. De...

The effect of acute administration of captopril, an angiotensin converting enzyme inhibitor, on vascular responses of rings of rat aortic smooth muscle was tested in vitro. Dose-response curves for various vasoactive agents were obtained before and after exposure to captopril (2 x 10(-4) M) for 30 minutes. In the presence of captopril, contractile responses to angiotensin I (5 x 10(-10) to 5 x ...

We developed a packed-column chromatographic procedure capable of simultaneous quantitation of methanol, ethanol, isopropanol, acetone, and ethylene glycol. This method was then updated to a rapid, sensitive, wide-bore capillary method. The packed-column system uses direct injection of 1 microL of Na2WO4/H2SO4-deproteinized serum onto a 1.8 m x 2 mm (i.d.) column packed with 80/100 HayeSep R. A...

Polypeptide constituents of the lens glucose transporter have been identified by photoaffinity labelling with cytochalasin B. The urea-insoluble fraction of monkey lens was irradiated at 280 nm for 30 min in the presence of 5 X 10(-7) M 3H-cytochalasin B. After extensive washing, the membranes were solubilized and their polypeptide composition determined by SDS-PAGE. Radioactivity was extracted...

L-NAME (1 10 mM, 30 min of incubation) potentiates the tension developed (Td) in the isolated rat hemidiaphragm stimulated by direct subtetanic electrical stimulation (DSES) (F = 14 Hz). The aim of our study was to investigate the possible modulatory effect of local anaesthetic (lidocaine) and beta adrenergic blockers, propranolol and atenolol, on the L-NAMEinduced potentiation of isometric con...

Accumulation of cyclic AMP was studied in guinea pig cerebral slices when Na+ levels in the bathing medium were varied or agents which affect tissue Na+ content were added. When NaCl was gradually replaced with Tris-HCl or choline chloride, cyclic AMP formation was progressively enhanced. ;when Na+ was below 30 mM, cyclic AMP formation reached the maximum (approximately 30 fold), but this incr...