Phenolics in the Chinese olive (Canarium album (Lour.) Raeusch) fruit significantly affect its flavor and quality. The shikimate pathway is a bridge connecting primary metabolism secondary through which fixed carbon can be transformed into phenolics. In this study, we aimed to reveal relationship between phenolic compound biosynthesis. Three fruits (cv. Tanxiang (TX), Changying (CY) Lingfeng (L...

Glucose pulse experiments were performed to elucidate their effects on the carbon flux into the aromatic amino acid pathway in different Escherichia coli strains. Using a 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP, aroB(-))-producing strain, a fed-batch fermentation strategy specialized for glucose pulse experiments was developed and further applied for 3-dehydroshikimate (DHS, aroE(-))-...

Coding regions of a cDNA for precursor and mature chorismate synthase (CS), a plastidic enzyme, from Corydalis sempervirens were expressed in Escherichia coli as translational fusions to glutathione-S-transferase. Fusion proteins were purified, and precursor and mature forms of CS were then released by proteolytic cleavage with factor Xa. Although mature CS was enzymatically active after releas...

The 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19) is a key enzyme in the shikimate pathway for the production of aromatic amino acids and chorismate-derived secondary metabolites in plants, fungi, and microorganisms. It is also the target of the broad-spectrum herbicide glyphosate. Natural glyphosate resistance is generally thought to occur within microorganisms in a strong s...

The enzyme 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase (EC 4.1.2.15) catalyzes the first committed step in the shikimate pathway of tryptophan synthesis, an important precursor for the production of terpenoid indole alkaloids (TIAs). A full-length cDNA encoding nuclear coded chloroplast-specific DAHP synthase transcript was isolated from a Catharanthus roseus cDNA library. This ...

A suspected glyphosate-resistant goosegrass [Eleusine indica (L.) Gaertn.] population, found in Washington County, Mississippi, was studied to determine the level of resistance and whether the resistance was due to a point mutation, as was previously identified in a Malaysian population. Whole plant dose response assays indicated a twoto four-fold increase in resistance to glyphosate. Leaf disc...

In 2003, a glyphosate-resistant plantago (Plantago lanceolata L.) population located in the Robertson district of South Africa was subjected to different glyphosate dosages and highest dosage (7200 g a.e. ha−1) gave no acceptable levels control. Here we reconfirm resistance investigate mechanism resistance. Dose-response curves indicated that rate causing 50% survival (LD50) for resistant (R) b...

The shikimate pathway has been described as a metabolic tree with many branches that led to the synthesis of an extensive range of products. This pathway is present only in bacteria, fungi, and plants. While there is only little difference in the sequence of the chemical reactions of the pathway, significant differences exist in terms of organization and regulation. In the main trunk of the shi...

site-directed mutagenesis (sdm) as a powerful technique was used to change two important and conserved amino acids in 5-enolpyruvylshikimate 3- phosphate synthase (epsps) gene of e. coli. the mutations changed glycine 96 to alanine and alanine 183 to threonine. these two amino acids are very important for intraction of the wide spectrum herbicide, glyphosate, to epsp synthase enzymes. by design...

The catalytic mechanism of 3-deoxy-D-manno-2-octulosonate-8-phosphate (Kdo8P) synthase from Escherichia coli was investigated under pre-steady-state conditions using rapid chemical quench flow methods. The results suggest the formation of acyclic bisphosphate 1 as a reaction intermediate. © 1997 Elsevier Science Ltd. 3-Deoxy-D-manno-2-octulosonate-8-phosphate (Kdo8P) synthase (EC 4.1.2.16) is a...