In order to test the metabolic stability of 9-substituted cytokinins, 6-benzylamino-9-methyl purine has been synthesized and labeled with (14)C in the 9-methyl carbon or doubly labeled with (14)C in the 9-methyl carbon and (3)H in the methylene moiety of the side chain. Although the 6-benzylamino-9-methylpurine is chemically stable, cytokinin-requiring tissues begin removing the 9-substituent i...

Aromatic cytokinins (ortho-topolin riboside, 6-benzylaminopurine riboside and 6-(2-hydroxy-3-methoxybenzyla mino)purine riboside) were tested for their possible interaction with human liver microsomal cytochromes P450 by absorption difference spectroscopy. All three compounds were shown to bind to the CYP enzymes producing a high to low spin shift of the heme iron yielding a Soret absorption ba...

Aim. The effect of chronic ultraviolet B (UV-B) radiation and cytokinin benzylaminopurine (6-BAP) on the growth, content photosynthetic pigments hydrogen peroxide (HP) in leaves pea plants (Pisum sativum L.) was studied. Methods. Pea cultivar Gotivsky were irradiated by UV-B at a dose 3.5 kJ/m2 per day during 5 days. Groups non-irradiated treated with 6-BAP after end radiation. Length shoots ro...

6-Benzylaminopurine is a broad-spectrum plant growth regulator, able to rejuvenate plants, bring plants out of dormancy, cause the formation lateral shoots and root shoots, stimulates chlorophyll positively affects photosynthesis, making leaf darker greener, stronger, in many crops are able, with out-of-root processing green fruits berries, somewhat postpone ripening time favor size weight. The...

In this paper we investigated the effect of some phytohormones on the production of flavonoids in cell suspension cultures of Digitalis lanata. There were used two cell lines and different combinations and concentrations of phytohormones. The highest flavonoids amounts were obtained after 14 days of treatment. Among the tested combinations, the highest yield of flavonoids, 7.58 mg/g d.w. (dry w...

Apical meristems, first, second and third-fourth axillary buds of Ludwigia repens were cultured on Murashige and Skoog (MS) medium containing various concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ) and αnaphthaleneacetic acid (NAA) for micropropagation. Micropropagation was best achieved from apical meristem on MS medium containing 0.05 mg dm TDZ and 0.1 mg dm NAA. It was noted t...

An effi cient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortifi ed with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of hig...