Susceptibility to protoxin and toxin forms of Cry1Ab and the binding of (125)I-labeled Cry1Ab and Cry1Ac has been examined in three Plodia interpunctella colonies, one susceptible (688(s)) and two resistant (198(r) and Dpl(r)) to Bacillus thuringiensis. Toxicological studies showed that the 198(r) colony was 11-fold more resistant to Cry1Ab protoxin than to Cry1Ab activated toxin, whereas the D...

The initial antibody repertoire is determined by multiple heavy and light chain variable region (V. and VL) ~ gene segments and multiple joining gene segments (J~, Jx, JH, and D.). These gene segments can join in various combinations (1-8) and complete V gene products can associate in different combinations. As a result of combinational joining and association, the size of the preimmune reperto...

A bioflocculant-producing bacterium, Bacillus megaterium SP1, was isolated from biofloc in pond water and identified by using both 16S rDNA sequencing analysis and a Biolog GEN III MicroStation System. The optimal carbon and nitrogen sources for Bacillus megaterium SP1 were 20 g L-1 of glucose and 0.5 g L-1 of beef extract at 30°C and pH 7. The bioflocculant produced by strain SP1 under optimal...

Alcohol Dehydrogenase (ADH) gene was isolated from Bacillus subtilis by PCR amplification. The 1.1kb amplified fragment was confirmed to be the ADH gene by DNA sequencing. The gene was inserted into expression plasmid pYC6/C7, transformation HD34 beer yeast, as well as some transformants with ADH activity were obtained. [Nature and Science, 2004,2(2):64-66]

The 64-kDa membrane protein of Bacillus subtilis is evidently involved in the attachment of secreting ribosomes to membrane. On immunoprecipitation with antibody to this protein, the solubilized particulate fraction, with or without prior chemical cross-linking, yields a complex of four proteins (64, 60, 41, and 36 kDa). This "S complex" was found to be associated with membrane-free ribosomes r...

We report here the draft genome sequence of Bacillus respiratorii VT-16-64, a novel spore-forming bacterium isolated from the bronchiolar alveolar lavage fluid of a patient with chronic obstructive pulmonary disease. The genome was comprised 4,831,386 bp with 4,399 predicted protein-coding genes, including those associated with antibiotic resistance and virulence.

Address for correspondence: Liapakis George, Department of Pharmacology, Faculty of Medicine, University of Crete, Voutes, Heraklion, 71003, Crete, Greece, Tel.: 30-281-0394-525, Fax: 30-281-0394-530, e-mail: [email protected] Fahmy Hesham, Department of Pharmaceutical Sciences, College of Pharmacy, South Dakota State University, Brookings, SD 57007, USA, Tel.: +1-605-688-4243, Fax: +1605-688...