Patients and methods Fresh tumour samples from 27 CRC patients were used to prepare tumour spheroids. Autologous PBMCs were isolated by ficoll density gradient. Tumour spheroids co-cultured with or without PBMCs were treated with catumaxomab for 96h. The cellular composition of the spheroids and the therapeutic impact on epithelial cells and leukocytes were measured by FACS analysis. The metabo...

hematological parameters are suitable biomarkers for evaluating the potential risk of the chemicals. the aim of this study was to investigate of acute crude oil exposure on basic physiological functions of persian sturgeon, acipenser persicus. 210 juvenile persian sturgeon (9.4 ± 1g) were supplied by the rajaei fish farm in mazandaran province, iran.  juveniles were exposed to the crude oil (15...

The effect of concurrent degradation of tetrabromobisphenol A (TBBPA) by the strain Ochrobactrum sp. T under aerobic condition was investigated. The results demonstrated that four extra energy source-addition systems still followed pseudo-first order kinetics. The addition of ethanol or glucose could promote the biodegradation ability of Ochrobactrum sp. T to TBBPA, and 90.1 percent and 77.5 pe...

Recent studies have shown that UVB irradiation induces primary and secondary hyperalgesia in rats and humans peaking about 24h after UVB exposure. In the present study we investigated the changes in galanin, substance P and c-fos immunoreactivity in rat DRG and spinal cord at the L5 level 2-96h after UVB irradiation. UVB irradiation of the heel area in rats almost increased the skin blood flow ...

The aim of this work was to investigate the effect of different concentrations of NaCl (0 mM, 100 mM, 200 mM and 300 mM) on germination, seedling growth and acid phosphatase activity of Linum usitatissimum seeds. Flax seeds were germinated under control and saline (100, 200 and 300 mM NaCl) conditions and the effect of salt stress on germination and enzyme activity was determined. Final germina...

In this study the LC50 96h values of water-soluble fractions (WSF) of the Caspian Sea ? exploited crude oil were determined in beluga, Huso huso fingerlings. The fingerlings averaged 2.42 ? 0.11 g in weight were exposed to WSF crude oil at concentrations 24.75, 27, 29.25, 31.5, 33.75, 36, 38.25 ppm. The treatments were performed in three replicates and all changes in the specimens were determin...

the need of aquarists for proper and safe disinfection of fish with chloramine-t (cl-t) necessitates toxico/pathologic examinations.   this study assays toxicity level of cl-t and histopathologic changes of gills, liver, kidney and brain of treated zebrafish (danio rerio). groups of 10 zebrafish were treated with 0 (control), 5, 10, 15, 30, 50,100 and 200 mg/l of cl– t. mortality was recorded a...

Extensive evidence indicates that epinephrine (EPI) modulates memory consolidation for emotionally arousing tasks in animals and human subjects. However, previous studies have not examined the effects of EPI on consolidation of recognition memory. Here we report that systemic administration of EPI enhances consolidation of memory for a novel object recognition (NOR) task under different trainin...

Although gasoline is an important contaminant of aquatic ecosystems, information concerning the potential effects of this petroleum derivative on the DNA of aquatic biota is lacking. The present study aimed to evaluate the genotoxic and mutagenic effects of acute exposures (6, 24, and 96h) to gasoline water-soluble fraction (GWSF), diluted to 5%, on the Asian clam Corbicula fluminea. The comet ...