BACKGROUND Varicella zoster virus (VZV) causes chickenpox in children and zoster (zona) in the elderly. Using RFLP-PCR method for detection of VZV specific SNPs ORF38, 54 and 62 could distinguish the profile of VZV isolates. The aim of this study was to investigate enzymatic digestion pattern of VZV ORF38 and ORF54 in chickenpox patients using RFLP technique. METHODS Thirty-eight chickenpox p...

BACKGROUND Cytochrome P450 2E1 (CYP2E1) might be involved in the development of bladder cancer. However, previous studies of any association between CYP2E1 RsaI/PstI polymorphism and bladder cancer risk have yielded conflicting results. In this study, we performed a more precise estimation of the relationship by a meta-analysis based on the currently available evidence from the literature. ME...

Pancreatic secretory trypsin inhibitor (PSTI), a specific trypsin inhibitor secreted by acinar cells of pancreas, also acts as a growth factor. PSTI in various cancers has been reported to be associated with tumor invasiveness and pathological malignancy; however, such studies in urological cancers have not yet been performed. We therefore examined the expression of PSTI in fresh frozen specime...

Using polymerase chain reaction, we detected the varicella-zoster virus genome in the vitreous humor of two patients with clinically diagnosed acute retinal necrosis. One of the two cases was thought to be caused by infection with a varicella-zoster virus lacking a PstI cleavage site. We could not find any clinical differences between the two substrains. The presence of a PstI cleavage site on ...

INTRODUCTION: Several RFLFs have been described in the human phosphoglycerate kinase 1 (PGK1) gene in Xql3. Whereas the PstI polymorphism as detected with PGK1 cDNA (Hutz et al.) was complicated by multiple X-linked and autosomal constant bands, only two bands are seen with a fourth-intron genomic probe. We have compared this PstI RFLP to the 5' Bgll RFLP detected by pSPT-PGK (Vogelstein et al....

The aim of this study was to explore the cytochrome P450 2E1 (CYP2E1) RsaI/PstI polymorphism and risk of esophageal cancer (EC) in mainland Chinese populations. A systematic search of PubMed, EMBASE, Web of Science, CBM, CNKI and VIP databases for publications on the CYP2E1 RsaI/PstI polymorphism and risk of EC was performed. and the genotype data were analyzed in a meta-analysis. Odds ratios (...

Polymorphism. PstI restriction fragment length polymorphism (RFLP) in the ovine Aconitase 2 (AC02) gene. Source and Description of Probe. The probe is a .9kb AC02 cDNA from a porcine myocardial hgt 10 library (Zheng et al., 19901, cloned into the EcoRI site of pUC118. The insert was gel-purified and radiolabeled with [ c Y ~ ~ P I ~ C T P by the random primer method to specific activity > lo9 d...

INTRODUCTION In mammalian pancreatic cells, the pancreatic secretory trypsin inhibitor (PSTI) prevents the premature activation of digestive enzymes and thus plays an important role in a protective mechanism against tissue destruction by autophagy, a process which may ultimately cause diseases such as pancreatitis and pancreatic cancer. Insects, however, lack a pancreas and so far no PSTI-like ...