Two Capillary Zone electrophoresis (CZE) methods were developed in this study, one using an acidic buffer (sodium citrate pH = 2.5) and the other using a basic buffer (sodium tetraborate pH = 9.8). The two methods were compared on the basis of repeatability and reproducibility of results and the CZE method developed with the basic buffer was then selected for further studies. The method was ful...

Two Capillary Zone electrophoresis (CZE) methods were developed in this study, one using an acidic buffer (sodium citrate pH = 2.5) and the other using a basic buffer (sodium tetraborate pH = 9.8). The two methods were compared on the basis of repeatability and reproducibility of results and the CZE method developed with the basic buffer was then selected for further studies. The method was ful...

Here, we describe a novel reliable method to assess the significance of individual mutations within the thymidine kinase (TK) gene of herpes simplex virus type 1 (HSV-1) to nucleoside analogue resistance. Eleven defined single nucleotide polymorphisms that occur in the TK gene of clinical HSV-1 isolates and a fluorescence reporter were introduced into the HSV-1 strain 17(+) that had been cloned...

BACKGROUND Genital infection with herpes simplex virus (HSV) is common and can cause severe morbidity, over many years in some cases. Aciclovir provides suppressive therapy but there is debate over the effects of its use on the spread of infection. OBJECTIVES To explore the influence of the natural history of genital HSV and the impact of antiviral therapy. METHODS A simple mathematical mod...

A randomized prospective double-blind study was performed to compare the efficacy of a single 5 min 1072 nm narrow waveband light application against topical aciclovir applied five times daily in the treatment of herpes labialis. Treatment was initiated within 36 h of the onset of symptoms and the end point was defined as the day that the crust was discarded leaving an uninterrupted underlying ...

two capillary zone electrophoresis (cze) methods were developed in this study, one using an acidic buffer (sodium citrate ph = 2.5) and the other using a basic buffer (sodium tetraborate ph = 9.8). the two methods were compared on the basis of repeatability and reproducibility of results and the cze method developed with the basic buffer was then selected for further studies. the method was ful...

Eighteen psychotic patients meeting the DSM-IIIR criteria for schizophrenia and manic-depressive psychosis were treated with antiviral drugs-aciclovir and interferon or aciclovir aloneplus the conventional treatment for more than eight months. Before treatment was begun, a biological test (BT) was performed on blood and cerebrospinal fluid, using immunoelectromicroscopic techniques, and the tes...

Resistance to aciclovir (ACV) among Herpes simplex virus (HSV) isolates is increasingly being reported in the literature particularly in immunocompromised patients. However, there is only limited data available from India despite widespread use of ACV in our hospital. A cross-sectional study was hence conducted to determine the aciclovir (ACV) susceptibility of HSV 1 and 2 isolates using a dye ...