background ghrelin, a 28 amino acid peptide, is effective in control of appetite and body weight. acylated ghrelin peptide is the active form of this peptide which plays a major role in the body’s energy balance. objectives this study aimed to investigate the possible effect (s) of intensive resistance exercise on acylated ghrelin, growth hormone, glucose, insulin, and cortisol plasma levels. p...

There has been an increasing demand for diverse and more functional plant-based protein ingredients food uses. This study aims to improve the properties of pea isolate through acylation and/or conjugation with guar gum investigate physicochemical characteristics modified proteins. Acylated proteins were prepared by reacting acetic anhydride (AA) or succinic (SA) at 0.3 0.6 g AA SA per protein, ...

Yersinia pestis is an important human pathogen that is maintained in flea-rodent enzootic cycles in many parts of the world. During its life cycle, Y. pestis senses host-specific environmental cues such as temperature and regulates gene expression appropriately to adapt to the insect or mammalian host. For example, Y. pestis synthesizes different forms of lipid A when grown at temperatures corr...

Fatty-acylated sericin {1:0.7 molar ratio of sericin (Mr 18,700) to oleic acid} was prepared by lipase-catalyzed solid-phase synthesis in n-hexane containing oleic acid to endow sericin with interfacial properties. Acylation with oleic acid was confirmed by 1H-NMR. The fatty-acylated sericin exhibited superior emulsifying activity index and emulsion stability in the presence of 0-0.5 M NaCl, in...

The aim of the present study was to investigate the postprandial effect of diet composition on circulating acylated ghrelin levels in healthy women. A randomized cross-over study of three experimental treatments was performed. A total of 11 healthy young women of normal body weight completed the study. All 11 subjects consumed three iso-energetic meals of different macronutrient composition, a ...

The aim of this study was to investigate the differences in associations of serum acylated and des-acylated ghrelin, peptide YY (PYY) and leptin levels with physical activity (PA) and cardiorespiratory fitness (CReF) in adolescent boys (mean age of 14.0 years) with overweight (OWB; n=55) and with normal weight (NWB; n=154). METHODS Total PA was measured by 7-day accelerometry (counts/min) and...

Aminoglycosides are broad-spectrum antibiotics commonly used for the treatment of serious bacterial infections. Decades of clinical use have led to the widespread emergence of bacterial resistance to this family of drugs limiting their efficacy in the clinic. Here, we report the development of a methodology that utilizes aminoglycoside acetyltransferases (AACs) and unnatural acyl coenzyme A ana...

BACKGROUND Acylated ghrelin, biologically active form of ghrelin, activates growth hormone (GH) secretagogue receptor 1a and play a role in regulating of energy balance. The purpose of this study was to survey relationships between acylated ghrelin with GH, insulin resistance, lipid profile, and cardio respiratory function in lean and obese men. METHODS Nineteen obese men (body mass index 31....

S-acylation/deacylation cycles and vesicular transport are critical for an adequate subcellular distribution of S-acylated Ras proteins. H-Ras is dually acylated on cysteines 181 and 184, but it is unknown how these residues individually contribute to H-Ras trafficking. In this study, we characterized the acylation and deacylation rates and membrane trafficking of monoacylated H-Ras mutants to ...

The observation that chloroacetyl-m-alanine is asymmetrically hydrolyzed by crude kidney preparations at pH 7 (1) led to the development of a general and simple procedure for the resolution of racemic amino acids (2-12). This procedure was based upon the action of a concentrated enzyme preparation obtained from hog kidney which asymmetrically hydrolyzed most N-acylated racemic amino acids, and ...