Previous studies in our laboratory (1) have shown that intact ascites cells incorporate nicotinamide-14C into both nicotinamide adenine dinucleotide and nicotinic acid adenine dinucleotide. It was also shown that the specific activity of the nicotinamide adenine dinucleotide increased with increasing concentrations of nicotinamide-14C. High levels of nicotinic acid-r4C, on the other hand, did n...

The behaviour of adenine and 2’-deoxyadenosine-5’-monophosphate (dAMP) at positive surface potentials of a silver working electrode was investigated using surface-enhanced Raman scattering (SERS). The use of positive potentials in the presence of adenine or dAMP leads to a rapid accumulation of an intense spectrum. It is proposed that complexes of adenine (dAMP) with silver generate the observe...

An orphan G protein-coupled receptor from the rat has recently been demonstrated to act as a transmembrane receptor for the nucleobase adenine. The receptor is possibly involved in nociception. Here we report the cloning and functional expression of an additional G(i)-coupled receptor for adenine (Genbank accession code DQ386867). mRNA for this receptor was obtained from mouse brain and the mou...

Cobalt, nickel and copper complexes of adenine and guanine, as nucleic-acid constituents, were prepared. The adenine and guanine complexes are of tetrahedral and octahedral geometries, respectively. All are of high spin nature. The nickel complexes are of 2:1 metal:ligand ratio with Ni...Ni direct interaction in the guanine complex. The coordination bonds of adenine metal complexes are calculat...

1. S-Adenosylhomocysteinase (S-adenosylhomocysteine hydrolase, EC 3.3.1.1) was slowly inactivated in the presence of adenine and adenine nucleotides (Ueland & Saebø, 1979b). 2. The enzyme was stabilized by 2-mercaptoethanol and dithiothreitol, and was slowly inactivated at 37 degrees C in the absence of reducing agents and rapidly inactivated in the presence of 5,5'-dithiobis-(2-nitrobenzoic ac...

Hyperphosphatemia in chronic kidney disease (CKD) patients is a risk factor for cardiovascular events, progressive kidney failure, and mortality. Improved therapeutic interventions to control hyperphosphatemia depend greatly on robust animal models that recapitulate the CKD disease process. Murine-based models of CKD as compared to rat models present significant advantages due to available gene...

Abstract Background and Aims Kidney fibrosis is the hallmark of chronic kidney disease (CKD) progression, whereas no effective anti-fibrotic therapies exist. We previously reported that natural flavonol fisetin alleviated septic acute injury protected against hyperuricemic nephropathy in mice. However, versatile role potential mechanism fibrotic have not been well characterized. Recently, tubul...

The hydration of biomolecules is vitally important in molecular biology, so in this paper thesolvation energy and radial distribution function of DNA bases have been calculated by theMonte Carlo simulation.The geometries of isolated Adenine, Guanine, Cytosine, and Thyminehave been optimized using 6-31+G(d,p) basis function sets. These geometries then will be used inthe Monte Carlo calculation o...

Catalytic hydrogenation of the appropriate pyridine nucleotide coenzymes and their derivatives over palladium supported on barium carbonate in aqueous solution has yielded the following representatives of two novel series of coenzyme analogues: tetrahydronicotinamide adenine dinucleotide, tetrahydronicotinamide mononucleotide, 3-acetyltetrahydropyridine adenine dinucleotide, hexahydronicotinami...