The effect of physiological concentrations of caffeine (purified from Persian tea) on adenosine deaminase (ADA) activity at physiological and pathological concentrations of adenosine (as substrate) in 50 mM Tris-HCl buffer (pH 7.3) at 37°C was investigated, using UV-VIS spectroscopy. ADA exhibited a bi-phasic activity behavior and both phases showed positive cooperativities indicating adenosine...

The thermal denaturation of adenosine deaminase (ADA) has been investigated in the presence of sodium n-dodecyl sulphate (SDS) over the temperature range of (293-363K) in 2.5 mM phosphate buffer, pH 6.4 by temperature scanning spectroscopy. The interaction of SDS caused the folding of adenosine deaminanse resulting in a decrease of TH (temperature of minimum solubility), TS<...

the thermal denaturation of adenosine deaminase (ada) has been investigated in the presence of sodium n-dodecyl sulphate (sds) over the temperature range of (293-363k) in 2.5 mm phosphate buffer, ph 6.4 by temperature scanning spectroscopy. the interaction of sds caused the folding of adenosine deaminanse resulting in a decrease of th (temperature of minimum solubility), ts (temperature of maxi...

the effect of physiological concentrations of caffeine (purified from persian tea) on adenosine deaminase (ada) activity at physiological and pathological concentrations of adenosine (as substrate) in 50 mm tris-hcl buffer (ph 7.3) at 37°c was investigated, using uv-vis spectroscopy. ada exhibited a bi-phasic activity behavior and both phases showed positive cooperativities indicating adenosine...

In this study the effect of adenosine and caffeine on spontaneous activity of paragigantocellularis (PGi) neurons was investigated. The spontaneous activity of PGi neurons was significantly decreased by microinjection of adenosine (10 nM, 0.5 µl) into PGi nucleus of both control and morphine-dependent rats. The decrease in firing rate of PGi neurons of morphine-dependent rats was greater than t...

background: we report the analgesic efficacy of lumbar epidural adenosine injection in reducing the chronic neuropathic pain in a patient with primitive neuroectodermal tumor. report of the case: in this case report a 13-year-old male with primary neuroectodermal tumor (pnet) was administered in epidural space with adenosine to reduce his pain. the patient received 10ml of 0.25% ropivacaine and...

background in spite of recent advents, and novel improvements in the diagnosis and treatment of pulmonary tuberculosis (tb), definitive diagnosis of pulmonary tb in children is problematic. therefore, measurement of the serum level of adenosine deaminase (ada) was later introduced as a helpful marker for the diagnosis of tb. objectives the purpose of this study was to determine the usefulness o...

Previously, we have reported that the coronary reactive hyperemic response was reduced in adenosine A2A receptor-null (A2AAR?/?) mice, and it reversed by soluble epoxide hydrolase (sEH) inhibitor. However, is unknown aortic vascular response, therefore, hypothesized A2AAR-gene deletion mice affects adenosine-induced increase sEH A1 receptor (A1AR) activities. A2AAR?/? showed an sEH, AI AR CYP45...

HIGHLIGHTS • Adenosine induces a reduction in blood pressure, heart rate and renal flow. Caffeine (30 mg/kg) blocked all the cardiovascular changes induced by adenosine. The effect of caffeine lasts for 4 hours.