1 Construction of Libraries for Isolation of Adrenergic Receptor Genes Margaret A. Scofield, Jean D. Deupree, and David B. Bylund ........ 3 2 Isolation of Adrenergic Receptor Genes Margaret A. Scofield, Jean D. Deupree, and David B. Bylund ...... 37 3 Analyses of Adrenergic Receptor Sequences Jean D. Deupree, Margaret A. Scofield, and David B. Bylund ...... 53 4 Polymerase Chain Reaction Scree...

Pressure overload ventricular hypertrophy is accompanied by dysfunctional b-adrenergic receptor signaling due to increased levels of the b-adrenergic receptor kinase-1, which phosphorylates and desensitizes b-adrenergic receptors. In this study, we examined whether increased b-adrenergic receptor kinase 1 expression is associated with myocardial hypertrophy induced by adrenergic stimulation. Wi...

?-adrenergic receptor antagonists (?-blockers) are extensively used to improve cardiac performance in heart failure (HF), but the electrical improvements with these clinical treatments not fully understood. The aim of this study was analyze electrophysiological effects system remodeling reduced ejection fraction and underlying mechanisms. We a combined mathematical model that integrated signali...

A 5-trial inhibitory avoidance test using spontaneously hypertensive rat (SHR) pups has been used as an animal model of attention deficit hyperactivity disorder (ADHD). However, the roles of noradrenergic systems, which are involved in the pathophysiology of ADHD, have not been investigated in this model. In the present study, the effects of adrenergic α2 receptor stimulation, which has been an...

Heteronuclear NMR in combination with isotope labelling is used to study folding of polypeptides induced by metals the case metallothioneins, binding peptidic allosteric modulator ?-TIA human G-protein coupled ?1b adrenergic receptor, development therapeutic drugs that interfere biosynthesis outer membrane Gram-negative bacteria, and a system which protein assembly upon peptide addition. these ...

Introduction: Anxiety is among the most common and treatable mental disorders. Adrenergic and cannabinoid systems have an important role in the neurobiology of anxiety. The elevated plus-maze (EPM) has broadly been used to investigate anxiolytic and anxiogenic compounds. The present study investigated the effects of intraperitoneal (IP) injection of cannabinoid CB1 receptor antagonist (AM251) i...

The aim of the present study was to examine gene expression and protein concentrations of beta(1)- and beta(2)-adrenergic receptors in subcutaneous adipose tissue in obese subjects in response to weight loss. Eighteen obese subjects were studied during diet-induced weight loss. Beta-adrenergic receptor mRNA levels were quantified by reverse transcription-PCR-HPLC. Beta-adrenergic receptor prote...

We investigated the expression of a1-adrenergic receptor subtypes in intact human peripheral blood lymphocytes using reverse transcription–polymerase chain reaction (RT-PCR) and radioligand binding assay techniques combined with antibodies against the three subtypes of a1-adrenergic receptors (a1A, a1B, and a1D). RT-PCR amplified in peripheral blood lymphocytes a 348-bp a1A-adrenergic receptor ...

We investigated the expression of alpha1-adrenergic receptor subtypes in intact human peripheral blood lymphocytes using reverse transcription-polymerase chain reaction (RT-PCR) and radioligand binding assay techniques combined with antibodies against the three subtypes of alpha1-adrenergic receptors (alpha1A, alpha1B, and alpha1D). RT-PCR amplified in peripheral blood lymphocytes a 348-bp alph...

PURPOSE To investigate the role of β1-adrenergic receptors on insulin like growth factor (IGF)-1 receptor signaling and apoptosis in the retina using β1-adrenergic receptor knockout (KO) mice. METHODS Western blotting and enzyme-linked immunosorbent assay analyses were done on whole retinal lysates from β1-adrenergic receptor KO mice and wild-type littermates. In addition, vascular analyses o...