the physiological effects of a diet free of meat and eggs were studied. fifteen adult lacto-vegetarians were matched with non-vegetarian individuals by age, sex, body weight and height. a two-week dietary record was secured from each individual. the food consumed over a 48-hour period was measured. venous blood samples were taken from each individual for determination of serum total protein, ur...

217414_x_at HBA2 3040 hemoglobin, alpha 2 hemoglobin complex [SUMMARY:] The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes the following five loci: 5'zeta pseudozeta pseudoalpha-1 alpha-2 alpha-1 -3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but the...

In humans, embryonic, fetal, and adult hemoglobins are sequentially expressed in developing erythroblasts during ontogeny. For the past 40 years, this process has been the subject of intensive study because of its value to enlighten the biology of developmental gene regulation and because fetal hemoglobin can significantly ameliorate the clinical manifestations of both sickle cell disease and β...

A 4-yr-old boy was studied who placed to the left of the curve from showed typical findings of juvenile normal adult blood; the hemoglobin chronic myelogenous leukemia, inA2 level and the erythrocyte I antigen cluding massive hepatosplenomegaly, titer were reduced; and a structural thrombocytopenia, low leukocyte alkaanalysis of the ‘i-chain of the fetal line phosphatase, and absence of a hemog...

BACKGROUND Although erythroid cells prepared from fetal liver, cord blood, or blood from β-thalassemia patients are known to express fetal hemoglobin at high levels, the underlying mechanisms remain elusive. We previously showed that cyclic nucleotides such as cAMP and cGMP induce fetal hemoglobin expression in primary erythroid cells. Here we report that cAMP signaling contributes to high-leve...

Human hemolysate contains several minor components designated Hb A,,, Hb Alh, Hb A,,, which are post-translational modifications of the major hemoglobin component A,,. Individuals with diabetes mellitus have elevated levels of Hb A,,, a hemoglobin modified with a glucose moiety at the NH, terminus of each /3 chain. A new chromatographic technique using Bio-Rex 70 is described which not only all...

During erythropoiesis, hemoglobin (Hb) synthesis increases from early progenitors to mature enucleated erythrocytes. Although Hb is one of the most extensively studied proteins, the role of Hb in erythroid lineage commitment, differentiation, and maturation remains unclear. In this study, we generate mouse embryos and embryonic stem (ES) cells with all of the adult α and β globin genes deleted ...

The fetal-to-adult hemoglobin switch and silencing of fetal hemoglobin (HbF) have been areas of long-standing interest among hematologists, given the fact that clinical induction of HbF production holds tremendous promise to ameliorate the clinical symptoms of sickle cell disease (SCD) and β-thalassemia. In this article, we discuss historic attempts to induce HbF that have resulted in some ther...

It has been known that hemolysate is composed of not only hemoglobin but also non-hemoglobin proteins (NHP). NHP isolated effectively from hemolysate of adult and newborn by CM-Sephadex chromatography was investigated by polyacrylamide disc or slab electrophoresis. It was demonstrated on slab electrophoresis that adult NHP is composed of 4 zones (approximately 14 bands) with different mobilitie...

Bone marrow from mature goats and sheep was cultured in plasma clots, and three erythropoietin (ESF)-dependent responses-growth (colony formation), differentiation (globin production), and initiation of hemoglobin C (alpha2beta2C) synthesis--were quantitated. ESF concentrations below 0.01 U/ml supported colony growth and adult hemoglobin production in cultures of goat marrow, while maximal hemo...