In four experiments, the effects of pre-thaw delay, method of thawing, temperature of thawing solution and pellet volume on acrosome morphology and moti l i ty of" boar spermatozoa frozen by the pellet method were examined. Holding the frozen pellets for 0, 1.5, 3 or 4.5 min in a Styrofoam container before transferring them into the thawing solution resulted in maximum post-thaw percentages of ...

Objective. To determine the freezing and thawing rates necessary to maintain sperm viability during cryopreservation of Bocachico semen. Materials and methods. Four interactional treatments were implemented between two freezing (rapid and slow) and two thawing (rapid and slow) curves, in a 2x2 factorial as follows: rapid freezing-rapid thawing, rapid freezing-slow thawing, slow freezingrapid th...

In this paper, rapid freezing and thawing test of air-entrained fly–ash concrete was carried out. Gas adsorption of concrete pores, mass loss, dynamic elastic modulus, and chloride diffusion coefficient of concrete after different freezing and thawing cycles was tested; scanning electron image was used to study the microscopic pore structure of the specimens. Results show that after certain fre...

background: phospholipids are distributed asymmetrically between inner and outer leaflets of the plasma membrane of live cells. early during apoptosis, this asymmetry is disrupted and phosphatidylserine becomes exposed on the outside surface of the plasma membrane. there is little information about the effects of vitrification on apoptosis. objective: the aim of the present study was to evaluat...

Soil freezing-thawing cycle may substantially influence soil physical properties, microbial activity, and the rates of carbon and nitrogen cycling in soils. In this study, the soil water soluble organic C, N (WSOC, WSON) as well as microbial biomass C, N (MBC, MBN) of two alpine grassland types, alpine meadow and alpine steppe, were investigated after freezing-thawing cycles in a grassland land...

The motility of sperm after freezing and thawing is critical for effective cryopreservation. It is known that supplementation with cholesterol-loaded cyclodextrin (CLC) improves cryosurvival of sperm in various animals. To clarify the effects of supplementation with CLC on rabbit sperm motility after freezing and thawing, rabbit sperm motility was analyzed using a computer-assisted sperm analys...

Standardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10 spermato...

Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and DNA integrity of rat spermatozoa during semen freeze-tha...