Rabbits made unresponsive to BSA at birth were given two courses of immunization with various cross-reacting albumins at 3 months of age. Normal control rabbits, of equivalent age and weight, were similarly immunized. Sera obtained 7 days after the last injection were assayed for binding and precipitating antibody to six albumins and for their avidity for BSA. No significant differences were fo...

Background One of the major sources of hidden allergens in food is the use of spices that contain undeclared allergenic ingredients. Sesame and mustard are highly relevant in this context, as both seeds are frequently used in a variety of spices and can elicit severe allergenic reactions. Major allergens of mustard and sesame belong to the 2S-albumin family of seed storage proteins. 2S-albumins...

BACKGROUND Proteomic analysis is emerging as a highly useful tool in food research, including studies of food allergies. Two-dimensional gel electrophoresis involving isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis is the most effective method of separating hundreds or even thousands of proteins. In this study, albumin and globulin tractions of pea seeds cv. R...