A quantitative complement fixation test with influenza immune sera and virus antigens obtained from allantoic fluid is described. The method utilizes a photoelectric densitometer which provides a simple, objective, and accurate determination of the hemolytic reaction. The enhancement of the hemolytic activity of complement in the presence of serum or allantoic fluid necessitates a preliminary t...

During a study of the interference produced by heat-inactivated influenza virus with the growth of live virus in fragments of chick chorio-allantoic membrane it was found that following incubation of heated virus with membrane a new factor was released. This factor, recognized by its ability to induce interference in fresh pieces of chorio-allantoic membrane, was called interferon. Following a ...

In the present study, a recombinant adeno-associated virus vector containing the calcitonin gene related peptide gene (rAAV-CGRP) was constructed and the therapeutic effect of rAAV-CGRP on a chick umbilical artery vasospasm model induced by chick embryo allantoic cavity hemorrhage was investigated. Fresh specific pathogen-free fertilized chicken eggs were randomly divided into a rAAV-CGRP group...

The chick chorioallantoic membrane is a very simple extraembryonic membrane which serves multiple functions during embryo development; it is the site of exchange of respiratory gases, calcium transport from the eggshell, acid-base homeostasis in the embryo, and ion and H(2)O reabsorption from the allantoic fluid. All these functions are accomplished by its epithelia, the chorionic and the allan...

The lipid composition of the concentrated hemagglutininating active fraction (HF) of allantoic fluid from infected eggs, but free from Chlamydia psittaci 6BC, was compared to concentrated normal allantoic fluid (NAF). Phosphatidyl-choline (PC) and phosphatidylethanolamine were the major lipid classes of the total phospholipid fraction. Some quantitative differences were found in the amount of P...

Applicability of polymerase chain reaction (PCR) assay to detect Pasteurella multocida in experimentally infected embryonated chicken egg was assessed in the present study. PCR assay rapidly and specifically detected the genome of P. multocida in amniotic fluid, allantoic fluid and homogenates of infected embryo and its membranes. The sensitivity of detection was as low as 20 bacterial cells/ml...

A rapid and sensitive method for the detection and unambiguous typing of infectious bronchitis virus (IBV) is described. RNA was isolated from IBV-infected allantoic fluid and was transcribed into cDNA. This cDNA was amplified by the polymerase chain reaction. The polymerase chain reaction products were subsequently analyzed on an agarose gel. The presence of IBV-specific RNA in the allantoic f...

Newcastle disease virus (NDV) is a single-stranded, negative-sense RNA virus in the Paramyxoviridae family. Although primarily an avian pathogen, NDV is a potent oncolytic virus that has been shown to be safe and effective in a variety of preclinical cancer models and human clinical trials. To produce virus for oncolytic trials, NDV is commonly amplified in embryonated chicken eggs and purified...

The developing metanephric kidneys and chorioallantoic membrane (CAM) work in unison to ensure ion and water homeostasis in the avian embryo within its egg. This study focused on how avian renal structure and glomerular perfusion change in concert during development, as well as on changes in body fluid compartment osmolalities. White leghorn chicken eggs were incubated at 37.5°C and 55-60% rela...