Linkage disequilibrium (LD) mapping can be successful if there is strong nonrandom association between marker alleles and an allele affecting a trait of interest. The principles of LD mapping of dichotomous traits are well understood, but less is known about LD mapping of a quantitative-trait locus (QTL). It is shown in this report that selective genotyping can increase the power to detect and ...

The clustered regularly interspaced short palindromic repeats (CRISPR) system has been adopted for a wide range of biological applications including genome editing. In some cases, dissection of genome functions requires allele-specific genome editing, but the use of CRISPR for this purpose has not been studied in detail. In this study, using the p16INK4a gene in HCT116 as a model locus, we inve...

Objective(s): The population in Iran is a genetic admixture of the ancestral Aryan and other populations neighboring Iran. Different ethnic groups in Iran show wide regional distributions for many human leukocyte antigen (HLA) alleles. Therefore, it is necessary and sensible to study the differences in HLA allele distribution in different area. We studied the HLA class I and II allele frequenci...

Host plant resistance provided by solid stems has been the most effective means for mitigating wheat stem sawfly (WSS) (Cephus cinctus Norton) damage in spring and winter (Triticum aestivum L.). The trait originates from cultivar “Rescue” is associated with a quantitative locus allele Qss.msub-3BL.b that explains majority of variation solidness. Recently, new Qss.msub-3BL allele, designated Qss...

Abstract The pollination of self-incompatible diploid sweet cherry is determined by the S-locus alleles. We resolved S-alleles 50 cultivars grown in Estonia and their incompatibility groups, which were previously unknown for most tested cultivars. used consensus primers SI-19/20, SI-31/32, PaConsI, PaConsII followed allele-specific sequencing to identify S-genotypes. Surprisingly, 48% (24/50) c...

We describe here two alleles, an allele of the lambda1 locus present in the SJL strain (rlambda1lo) and an allele of the lambda1 locus present in the BALB/c strain (rlambda1 +), of a regulatory gene locus which specifically influences the expression of the mouse lambda1 light chain structural gene. The rlambda1 regulatory gene is not linked to either the major histocompatibility complex or to t...

Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction prevented. The self-incompatibility caused the functions of cell controlled genes called S or genes. results decreased pollination and ultimately yield loss. In apple (Malus domestica L.), multi-allelic S-locus. Approaches S-glycoprotein profiles allele-specific PCR methods using gene for ...

To investigate candidate genes that influence the risk of HTLV-I associated myelopathy/tropical spastic paraparesis (HAM/TSP), we analyzed 6 single nucleotide polymorphisms (SNP) in the interleukin-10 (IL-10) promoter region. METHODS: 280 cases of HAM/TSP patients and 255 HTLV-I seropositive asymptomatic carriers (HCs) from Kagoshima, Japan were studied. All subjects gave written informed conse...