We have detected a set of transcripts in Drosophila melanogaster cells which are homologous to repeating elements within the 'non-transcribed' spacer region of rDNA. The RNA molecules range from 240 to 1680 nucleotides, differing in length by an integral value of 240 nucleotides. We have sequenced several AluI fragments which characterise the main 240 nucleotide repeating element. We find that ...

The intermediate hosts of Schistosoma mansoni, in Brazil, Biomphalaria glabrata, B. tenagophila and B. straminea, were identified by restriction fragment length polymorphism analysis of the mitochondrial gene cytochrome oxidase I (COI). We performed digestions with two enzymes (AluI and RsaI), previously selected, based on sequences available in Genbank. The profiles obtained with RsaI showed t...

This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity...

background and objectives: sub-minimum inhibitory concentration (s-mic) can induce changes in morphology, virulence factors and biochemical properties ofbacterial pathogens. the goal of this study is to determine the sub-mic effect of ciprofloxacin and ampicillin on the haemotytic activity of e. coli. material and methods:two clinical isolates of e. coli with high heamolytic activity was select...

background and objectives: mycobacterium tuberculosis complex is consisted of homogenous organisms. they are slowly growing mycobacteria and their isolation and identification are difficult and time consuming. differentiation of mycobacterium bovis, causative mammalian tuberculosis, from other members of mycobacterium tuberculosis complex is very important in epidemiology and control of disease...

The aim of this study was to identify SNPs in leptin (LEP), leptin receptor (LEPR) and growth hormone (GH) genes in order to analyze genetic diversity of Slovak Spotted cattle. The total numbers of blood samples were taken from 353 Slovak Spotted cows originating from four farms. Genomic DNA was isolated by phenol-chloroform extraction method and analyzed by PCR-RFLP method. After digestion wit...