Ammonium serves as key nitrogen source and metabolic intermediate, yet excess causes toxicity. Ammonium uptake is mediated by ammonium transporters, whose regulation is poorly understood. While transport can easily be characterized in heterologous systems, measuring transporter activity in vivo remains challenging. Here we developed a simple assay for monitoring activity in vivo by inserting ci...

The inhibitory effect of ammonium on primary root growth has been well documented; however the underlying physiological and molecular mechanisms are still controversial. To avoid ammonium toxicity to shoot growth, we used a vertical two-layer split plate system, in which the upper layer contained nitrate and the lower layer contained ammonium. In this way, nitrogen status was maintained and onl...

INTRODUCTION  Fibrin tissue adhesive, which has applications in several areas of medicine, can be prepared by different methods. AIM  To compare fibrin tissue adhesives prepared by 3 different methods. METHOD  In this prospective experimental laboratory study, fibrin tissue adhesives prepared by the use of plasma fibrinogen (group 1), cryoprecipitation (group 2), and precipitation by ammoni...

T WO levels of ammonium chloride or of ammonium sulfate were added to the rations of commercial feeder cattle. The rations were made isonitrogenous, essentially isocaloric and to contain the same level of calcium, phosphorus and salt. Total gains were essentially the same for all lots, varying only 15.6 lb (7.1 kg) over a 112 day feeding period. Feed conversion was increased only on the ration ...

Lack of nitrogenous substrate and buffering capacity have been identified as causing failure in previous work on the treatment of fruit cordial wastewater using anaerobic continuous stirred tank reactors. In this study, ammonium bicarbonate was proposed to be used as the substrate for nitrogenous and buffering resources. In order to determine the toxicity effect of the ammonium salts on the ana...

Ammonium is an attractive nitrogen form for root uptake due to its permanent availability and the reduced state of the nitrogen. On the other hand, ammonium fluxes are difficult to control because ammonium represents an equilibrium between NH4+ and NH3, which are two N forms with different membrane permeabilities. There is increasing evidence that AMT-type ammonium transporters represent the ma...

PURPOSE To evaluate an intraocular drug delivery system consisting of the crystalline ammonium salt of 1-O-hexadecylpropanediol-3-phospho-ganciclovir (HDP-P-GCV) as a slow-release form of the drug. METHODS A dosage of 0.885, 1.57, 2.8, 4.486, or 8.85 micromol of ammonium salt HDP-P-GCV in 0.1 mL was intravitreally injected into rabbit vitreous. The toxicity and safety were evaluated with opht...