INTRODUCTION Diabetic foot ulcers (DFUs) are complex chronic wounds which have a major long-term impact on the morbidity, mortality and quality of patients. The objective of this study was to assess the efficacy and time sensitivity of human amnion/chorion membrane treatment in patients with chronic DFUs. METHODS The Cochrane Library, PubMed, Embase and Web of Science databases were systemati...

The first reported use of fetal membrane as skin substitute was by Davis in 1910. 1 In 1913, Salbella presented the first clinical report of successful use of amniotic membrane in the treatment of burns and skin ulcerations. 2 In 1940, DeRoth reported the use of amniotic membrane in the repair of conjuctival defects. From 1940 to 1965 a number of clinical trials of successful use of amniotic me...

Burns are one of the most widespread traumatic injuries. The number victims burns worldwide is steadily increasing. All this determines search for new highly effective methods treating burned patients. (1.2.4.5.20.)The main requirements wound healing creation an optimal microenvironment healing, high absorption capacity exudate, ability to prevent ingress microorganisms, sufficient permeability...

Guided bone regeneration is a new concept of large bone defect therapy, which employs a barrier membrane to afford a protected room for osteogenesis and prevent the invasion of fibroblasts. In this study, we developed a novel barrier membrane made from lyophilized multilayered acellular human amnion membranes (AHAM). After decellularization, the AHAM preserved the structural and biomechanical i...

PURPOSE To compare the effectiveness of standard revision surgery using mitomycin C (MMC) with revision using amniotic membrane transplantation and MMC for elevated intraocular pressure (IOP) after trabeculectomy. PATIENTS AND METHODS A retrospective, nonrandomized comparative study of 36 eyes of 36 patients with a failed trabeculectomy. Patients were divided into two groups, ie, a nonamnion-...

Background: Hemophilia B is an X-linked hereditary disorder of blood coagulation system which is caused by factor IX (FIX) deficiency. Factor IX is a plasma glycoprotein that participates in the coagulation process leading to the generation of fibrin. Replacement of factor IX with plasma-derived or recombinant factor IX is the conventional treatment for hemophilia B to raise the factor IX le...

An embryologist, as implied by his name, is primarily interested in the development of the embryo as a whole or at least in one or more of its intra-embryonic structures. Being a pathologist, whose interest in embryology has been stimulated by contact with the pathological problems of The Boston Lying-in Hospital, I have, perforce, become more interested in those extra-embryonic structures, the...

Gresser, Ion (Children's Hospital Medical Center, Boston, Mass.), Charles Chany, and John F. Enders. Persistent polioviral infection of intact human amniotic membrane without apparent cytopathic effect. J. Bacteriol. 89:470-475. 1965.-Poliovirus type I multiplied in organ cultures of the intact amniotic membrane. The behavior of the virus in this system differed markedly from that observed in m...

Human cytomegalovirus (CMV) is the most common cause of viral intrauterine infection in developed countries. It has been shown that CMV DNA was frequently detected in the fetal membranes when the placenta was infected in utero. However, it is still not clear whether CMV replicates in constituent cells of the fetal membranes. We investigated CMV infection of primary cultured chorion and amnion c...

Interstitial collagen gives fetal membranes tensile strength, and membrane rupture has been attributed to collagen degradation. A polymorphism at -1607 in the matrix metalloproteinase-1 (MMP-1) promoter (an insertion of a guanine (G)) creates a core Ets binding site and increases promoter activity. We investigated whether this polymorphism is functionally significant for MMP-1 expression in amn...