Peripheral blood leukemia cells from patients with acute monoblastic leukemia (AMoL) were tested for killer cell activity against target cells that detected natural killer cell-mediated or monocyte-mediated spontaneous cytotoxicity. The fibrosarcoma cell line Wehi 164, pretreated with actinomycin D to induce susceptibility to lysis, specifically detects the activity of unstimulated human monocy...

The monoclonal antibody solution hybridization assay is a novel enzyme immunoassay for detection of RNA with a biotinylated DNA probe. To increase the sensitivity of this test, a fluorescent substrate and an enzymatic amplification cycling system were compared with a conventional colorigenic substrate for alkaline phosphatase. The fluorescent, cycling, and colorigenic substrates detected, respe...

Leukemic blasts from patients with acute nonlymphoid leukemia were examined for the presence of Ig, receptors for IgGFc, and for their capacity to mediate antibody-dependent cellular cytotoxicity (ADCC) against chicken red blood cells (RBC) coated with IgG and spontaneous cell-mediated cytotoxicity (SCMC) against cells of K562 cell line. Leukemic blasts from acute myeloblastic leukemia (AML) pa...

We attempted to apply fluorescence resonance energy transfer technology to nucleic acid sequence-based amplification (FRET-NASBA) on the platform of the LightCycler system to detect Aspergillus species. Primers and probes for the Aspergillus 18S rRNA were newly designed to avoid overlapping with homologous sequences of human 18s rRNA. NASBA using molecular beacon (MB) showed non-specific result...

In this issue, Zou et al. report that vitamin C abrogated the ability of bortezomib (PS-341, Velcade) to induce apoptosis, G2-M arrest, and cleavage of caspase 8, caspase 3, and poly(ADP-ribose) polymerase and to augment tumor necrosis factor–related apoptosis-inducing ligand–induced apoptosis in various human cancer cell lines (1). Moreover, vitamin C suppressed bortezomib-mediated inhibition ...

a comparative study on patient satisfaction with hospital services in amol     nazari r* [1] , babaalipour mouziraji f [2] , sharif nia sh [3] , amini j [4] , akbari s [5]     received: 5 april, 2011 accepted: 19may, 2011    abstract:  background & aims: patient satisfaction is one of the main indicators for measuring effectiveness of health system. patient satisfaction in different areas is in...

Biological degradation of terrestrially derived macromolecules including lignin and cellulose has been shown to produce a large number of environmentally relevant phenolic compounds. It has demonstrated that extracellular superoxide (O2 ) is produced by heterotrophic bacteria that are common in lakes, soil, hydrothermal vents, marine sediments, estuaries and oceans. Rates of superoxide producti...

In Burkina Faso, the levels of plasma homocysteine (Hcy) are lower and the methionine loading tests suggest a more effective Hcy metabolism [1,2]. We found a relevant difference in the allele frequencies of C677T: T 7.7% in the young and 3.3% in old subjects respectively (see Table 1). Frequencies of C677T genotypes in old and young individuals were in Hardy Weinberg equilibrium (HWE). The diff...