substrates' concentration profile was studied in a porous matrix containing immobilized amyloglucosidase for glucose production. this analysis was performed by using an analytical method called least square method, and the results were compared with numerical solution. effects of effective diffusivity, michael's constant, maximum reaction rate and initial substrate concentration were studied on...

A high-performance liquid chromatographic method using refractive index detection for the determination of glycogen in sea urchin (Paracentrotus lividus) gonads is developed. After alkaline digestion with sodium carbonate, samples are adjusted to pH 4.6 with citric acid and incubated with amyloglucosidase to hydrolyze the glycogen. The resulting glucose is determined using a Spherisorb NH2 colu...