Subcellular localization of the starch-degrading enzymes in Vicia faba leaves was achieved by an electrophoretic transfer method through a starch-containing gel (SCG) and enzyme activity measurements. Total amylolytic and phosphorolytic activities were found predominantly in the extrachloroplastic fraction, whereas the debranching enzymes showed homogenous distribution between stromal and extra...

Eco-enzymes are one of the potential sources for obtaining amylolytic bacterial isolates. The study aims to screen bacteria from eco-enzymes, characterize semi-purification amylase, and identify molecularly using 16S rRNA gene. Screening was carried out by measuring index on a Nutrient agar medium containing 1% tapioca starch. isolate which had highest non-pathogenic selected amylase characteri...

Amylases are among the most important enzymes used in modern biotechnology particularly in the process involving starch hydrolysis. Fungal amylase has large applications in food and pharmaceutical industries. Considering these facts, endophytic fungi isolated from the plant Alpinia calcarata (Haw.) Roscoe were screened for amylolytic activity on glucose yeast extract peptone agar (GYP) medium. ...

Bestimmung der Glucoamylase in Kulturfiltraten, die andere amylolytische Enzyme enthalten. Es wird eine verbesserte Methode fur die bestimmung der Glucoamylase in Gegenwart anderer amylolytischer Enzyme vorgeschlagen. Sie beruht auf der Verwendung von Dextran mit niedrigem Molekulargewicht als spezifisches Substrat. Die Bestimmung der aktivitat erfolgt durch quantitative Ermittlung der Glucosem...

Amylolytic enzymes are most important in the biotechnology industries which are highly applied in food, fermentation, textile and paper industry. The enzyme is also used in starch liquefaction, brewing, pharmaceuticals, detergents, drugs, toxic waste removal and in oil drilling (Ajay & Fagade, 2003). In the present study isolation of a bacterial strain from soil samples was done and it's physio...