Analytic hierarchy process (AHP) is a utility theory based decision-making technique, which works on a premise that the decision-making of complex problems can be handled by structuring them into simple and comprehensible hierarchical structures. However, AHP involves human subjective evaluation, which introduces vagueness that necessitates the use of decision-making under uncertainty. The vagu...

background and objective: intraoperative consultation by frozen section is a high – risk procedure with important consequences. therefore, it is critical to determine efficiency of frozen section performance periodically. this study was performed to determine the accuracy of frozen section in urmia university of medical sciences. materials & methods:inthis cross sectional study, we compare...

background and objectives: hiv-1 and hcv infections are life threatening problems in patients who receive blood products. serological methods have proven useful in detecting these infections, but there are setbacks that make it challenging to detect these infectious agents. by the advent of nucleic acid testing (nat) methods, especially in multiplex format, more precise detection is possible. m...

As the development of life science and biomedical science, the detection of biomolecules with low abundance and the acquisition of ultra weak biological signals have been become a bottleneck of these fields. Therefore, developing high sensitive analytical methods coupling with the specificity of biological recognition evens is in urgent need [1]. However, owing to the inherent background signal...

We describe the development and analytical validation of a 7-plex polymerase chain reaction assay coupled to a bead-based liquid suspension array for detection of multiple ruminant Mycoplasma spp. The assay employs a combination of newly designed and previously validated primer-probe sets that target genetic loci specific for Mycoplasma bovis, Mycoplasma mycoides cluster, Mycoplasma mycoides su...

INTRODUCTION Lyme borreliosis (LB) is the most common tick transmitted disease in Europe. The diagnosis of LB today is based on the patient´s medical history, clinical presentation and laboratory findings. The laboratory diagnostics are mainly based on antibody detection, but in certain conditions molecular detection by polymerase chain reaction (PCR) may serve as a complement. AIM The purpos...

A sensitive monitoring of biological analytes, such as biomolecules (protein, lipid, DNA and RNA), and biological cells (blood cell, virus and bacteria), is essential to assess and avoid risks for human health. Nanobiosensors, analytical devices that combine a biologically sensitive element with a nanostructured transducer, are being widely used for molecular detection of biomarkers associated ...

Chromatin immunoprecipitation (ChIP) is a powerful assay used to probe DNA-protein interactions. Traditional methods of implementing this assay are lengthy, cumbersome and require a large number of cells, making it difficult to study rare cell types such as certain cancer and stem cells. We have designed a microfluidic device to perform sensitive ChIP analysis on low cell numbers in a rapid, au...

Early and accurate disease diagnosis still remains a major challenge in clinical settings. Biomarkers could potentially provide useful tools for the detection and monitoring of disease progression, treatment safety and efficacy. Recent years have witnessed prodigious advancement in biosensor development with research directed towards rapid, real-time, label-free and sensitive biomarker detectio...