Ten maize lines prevalently used by breeders in the maize program were screened for their ability to form type II callus. Mature seeds were used as explant for in vitro culturing. Inbred lines showed high embryogenic response and formed friable, type II calli. And also exhibited successful plant regeneration. Various culture media compositions based on Murashige and Skoog (1962) salts thus inve...

abstract to produce embryogenic callus and somatic embryo, whole flower explants were collected at two sampling stages of i, iii. to produce embryogenic callus and somatic embryo, ms medium supplemented with 5 and 10µm 2,4-d and 2µm bap were used. for embryo differentiation, ms medium with 0.5mg/l iba, ms medium without any plant growth regulators, ms medium with 2mg/l iba and 0.2mg/l bap, ms m...

Quantitative and qualitative levels of DNA methylation were evaluated in leaves and callus of Pennisetum purpureum Schum. The level of methylation did not change during leaf differentiation or aging and similar levels of methylation were found in embryogenic and nonembryogenic callus.

Wheat is notorious for callus induction, which is a major hindrance in direct gene transfer and consequently for genetic improvement programs. In order to provide a successful platform for gene transformation, good callus quantity and quality is important. We investigated the variation in callus induction capabilities of Pakistani wheat cultivars and measured the reducing sugar content in the i...

A method has been developed for plant regeneration by encapsulation of somatic embryos obtained from callus cultures derived from semi-mature cotyledon explants of Dalbergia sissoo Roxb. (family Fabaceae). Embryogenic callus was developed from cotyledon pieces on Murashige and Skoog (1962) medium supplemented with 9.04 μM 2,4dichlorophenoxyacetic acid and 0.46 μM kinetin. The somatic embryos we...

lilium ledebourii (baker) boiss. (liliaceae) is a critically endangered lily species native tonorthern iran, where it is protected by law. in order to develop a cost effective method for largescalepropagation, the effects of three culture systems (solid, liquid and temporary immersion)and two types of cytokinins [6-benzyladenine (ba) and thidiazuron (tdz)] were studied onthe in vitro plant rege...

Moso bamboo [Phyllostachys heterocycla var. pubescens (Mazel ex J. Houz.) Ohwi] is one of the most important forest crops in China and the rest of Asia. Although many sympodial bamboo tissue culture protocols have been established, there is no protocol available for plantlet regeneration as indicated by callus induction for monopodial bamboos, such as Moso bamboo. In the present report, embryog...

Orthosiphon stamineus is one of the most prominent medicinal herbs widely grown in Southeast Asia. Propagation O. using tissue culture technique helps to rapidly produce samples for research purposes, increase production secondary metabolites and considered as strategies plant genetic improvement. Despite vast information on pharmacological properties, protein profile this species currently und...

Picea mongolica is a rare tree species in China, which of great significance combating desertification and improving the harsh ecological environment. Due to low rate natural regeneration, high mortality, susceptibility pests cold springs, has gradually become extinct. At present, somatic embryogenesis (SE) most effective method micro-proliferation conifers, but induction embryogenic callus (EC...

Davidia involucrata Baill. (D. involucrata), a rare and endangered wild plant, is native to China globally recognized as an ornamental tree species. However, D. exhibits inherent biological characteristics that contribute its low reproductive efficiency. To address this challenge, somatic embryogenesis, biotechnological method, offers numerous advantages, including enhanced efficiency, large co...