We have cloned a novel krüppel-like transcription factor of Xenopus that encodes POZ/zinc finger protein by expression cloning. Overexpression of mRNA resulted in interference with gastrulation. Because the injected embryo looks like a mushroom in appearance at the neurula stage, we have named this gene champignon (cpg). In cpg-injected embryos, the blastopore appeared normally, but regressed t...

Background: The assisted reproductive techniques (ARTs) such as in vitro fertilization, embryo transfer and cryopreservation of gametes have considerably contributed to the development of biomedical sciences in addition to improved breeding in domestic animals and infertility treatment in humans. However, ARTs used in canine species have strictly limited utility when compared with other mammali...

background: previous studies have shown that morphine consumption during pregnancy may cause delay or defect of embryo development or abnormal nervous system function in the human and animal models. in the present study, the highest density of morphine accumulation in the central nervous system of rat embryos was evaluated using c14‑morphine. methods: female wistar rats (w 170‑200 g) used and w...

The objective of this retrospective study was to evaluate the factors influencing developmental stage of bovine embryos recovered from superovulated dairy cattle 7 d after estrus. From 217 superovulated dairy cows and heifers, 2,211 eggs were recovered, of which 1,495 were classified as transferable embryos based on morphological evaluation of developmental stage and quality. From the evaluated...

Studies of knockout (KO) mice with defects in the endolysosomal two-pore channels (TPCs) have shown TPCs to be involved pathophysiological processes, including heart and muscle function, metabolism, immunity, cancer, viral infection. With objective studying TPC2's roles for first time a large, more humanlike animal model, TPC2 KO pigs were produced using CRISPR-Cas9. A major problem CRISPR-Cas9...

Continuous improvements to assisted reproductive technology (ART) have facilitated the advancement and preservation of animal genetics as well as animal reproductive efficiency. One of the ART techniques is cryopreservation which facilitates embryo transfer procedures. In the present experiment embryos produced in vivo or in vitro were transferred to the recipient ewes just after collection (fr...

BACKGROUND Successful Embryo Transfer (ET) technique is a fateful step of all efforts to achieve live births from in vitro produced embryos in assisted reproductive techniques or in knockout, transgenic or cloned animal projects. Small reproductive tract of mice and limitation of current techniques may not well satisfy the requirements for mass production of genetically modified mice. Genetic a...

Background: Vitrification has been usually used as an assisted reproductive technology in animals and humans. This method needs high concentrations of cryoprotectants that can be toxic with high cooling degrees. Then, vitrification could be change histone modifications such as methylation and acetylation can performance as regulatory controls of gene transcription. So, the purpose of the presen...