Cell migration is crucial for processes such as immune defense, wound healing, or the formation of tumor metastases. Typically, migrating cells are polarized within the plane of movement with lamellipodium and cell body representing the front and rear of the cell, respectively. Here, we address the question of whether this polarization also extends to the distribution of ion transporters such a...

Laboratório Dra. Maria do Carmo Moreira de Souza, Departamento de Parasitologia, Instituto de Patologia Tropical e Saúde Pública, Rua Delenda Rezende de Melo, s/no, Setor Universitário, 74605-050 Goiânia, GO, Brasil *Laboratório de Biologia Molecular, Instituto de Ciências Biologicas **Laboratório de Pesquisa da Doença de Chagas, Hospital das Clínicas, Universidade Federal de Goiás, Goiânia, GO...

Basal HCO(3)(-) secretion across the duodenum has been shown in several species to principally involve the activity of apical membrane Cl(-)/HCO(3)(-) exchanger(s). To investigate the identity of relevant anion exchanger(s), experiments were performed using wild-type (WT) mice and mice with gene-targeted deletion of the following Cl(-)/HCO(3)(-) exchangers localized to the apical membrane of mu...

The recent introduction of miniature liquid ion-exchanger electrodes provides the possibility of overcoming previously encountered limitations on the measurement of specific ionic activities in biological fluids. Hydrogen ion and sodium ion activities have been stuccessfully determined by means of glass-membrane electrodes(l-5). However, in the presence of sodium, glass electrodes are usually i...

Measurements of cytosolic pH (pHi) 36Cl fluxes and free cytosolic Ca2+ concentration ([Ca2+]i) were performed in the clonal osteosarcoma cell line UMR-106 to characterize the kinetic properties of Cl-/HCO3- (OH-) exchange and its regulation by pHi and [Ca2+]i. Suspending cells in Cl(-)-free medium resulted in rapid cytosolic alkalinization from pHi 7.05 to approximately 7.42. Subsequently, the ...

Basolateral uptake of organic anions in renal proximal tubule cells is indirectly coupled to the Na(+) gradient through Na(+)-dicarboxylate cotransport and organic anion/dicarboxylate exchange. One member of the organic anion transporter (OAT) family, Oat1, is expressed in the proximal tubule and is an organic anion/dicarboxylate exchanger. However, a second organic anion carrier, Oat3, is also...