Cadmium at environmental concentrations is a risk factor for many diseases, including cardiovascular and neurodegenerative diseases, in which macrophages play an important role. The aim of this study was to evaluate the effects of cadmium at low environmental (nanomolar) concentrations on apoptotic processes in THP-1(acute monocytic leukemia cells line)-derived macrophages, with special focus o...

Purpose: To test the effect of Cryptotanshinone (CPT), a natural compound isolated from the plant Salvia miltiorrhiza Bunge, on human leukemic cell lines (HL-60). Methods: HL-60 cells were treated with CPT. Cell growth inhibition (%) was quantitated using MTT assay. Apoptosis detection with Annexin V-FITC/propidium iodide staining was followed by flow cytometry. Caspase-3, caspase-8, and caspas...

The present study compared the effect of resveratrol on HCT116 and Caco-2 human colon cancer cells. Annexin V/propidium iodide staining, MTT assay and western blot analysis revealed that resveratrol induced cycle arrest in the two cell lines, which was evidenced by cell cycle analysis and changes in the expression of the cell cycle proteins cyclin-dependent kinase (CDK) 2, CDK4, cyclin D1, prol...

In the present study, we investigated the effect of intracellular glutathione (GSH) depletion in heart-derived H9c2 cells and its mechanism. L-buthionine-S,R-sulfoximine (BSO) induced the depletion of cellular GSH, and BSO-induced reactive oxygen species (ROS) production was inhibited by glutathione monoethyl ester (GME). Additionally, GME inhibited BSO-induced caspase-3 activation, annexin V-p...

In a group of 117 patients with multiple myeloma (MM) examined at the time of diagnosis, i.e. excluding previous chemotherapy, we analysed the levels of propidium-iodide (proliferative) - PC-PI/CD(138) and annexin-V (apoptotic) - PC-AI/CD(138) indices of myeloma plasmocytes using the method of flow-cytometry to determine their relationship to prognosis. It was revealed that patients with high v...

The cytotoxic activity of natural killer cells is usually tested by radioactive assay CSI Cr release assay), which detects the release of cytoplasmic contents after plasma membrane disintegration of dying cells. In contrast to this indirect evaluation of cytotoxicity, the assessment of cell damage by flow cytometry aims to provide a more exact characterization of the death pathway via detec...

Fenbendazole (FBZ) is a commonly used anthelmintic in veterinary medicine that has recently been found to have anticancer effects humans. On the other hand, few studies examined anti-inflammatory of FBZ, and its mechanism unknown. In this study, mouse bone marrow cells (BMs) were treated with lipopolysaccharide (LPS), representative inflammation-inducing substance, generate situation similar os...

Background/Aim: We investigated the cytotoxic effects of plumbagin on metastatic retinoblastoma, using highly cell line Y79. Materials and Methods: Effect growth was assessed with water-soluble tetrazolium 1 (WST-1) proliferation assay automated hemocytometry trypan blue-exclusion assay. Cell death studied acridine orange/ethidium bromide live-dead annexin-V-fluorescein isothiocyanate/propidium...

many cellular damages either in normal or cancerous tissues are the outcome of molecular events affected by ionizing radiation. t‑cells are the most important among immune system agents and are used for biological radiation dose measurement in recommended standard methods. the herbs with immune modulating properties may be useful to reduce the risk of the damages and subsequently the diseases. ...

AbstractThe purpose of this investigation was to evaluate the antiproliferative and apoptogenic mechanistic studies of methanol extract of Anthocephalus cadamba (MEAC) on Dalton’s lymphoma ascites (DLA) cells treated mice. Determination of antiproliferative activity was performed by using different DLA cells (2×106 cells, i.p.) inoculated mice groups (n=12). Groups were treated for 14 consecuti...