The relationship between 7S anti-IgG and antibodies to streptococcal cell wall peptidoglycan was examined for four streptococcal Group C antisera. Homogeneous 7S anti-IgG components in these sera were isolated by means of an IgG immunoadsorbent column. For two of the four antisera, the anti-peptidoglycan activity of the 7S anti-IgG had specificity for the pentapeptide, L-Ala-D-Glu-gamma-L-Lys-D...

ed by: African Index Medicus (WHO), CAB Abstracts, Index Copernicus, Global Health Abstracts, Asian Science Index, Index Veterinarius, Bioline International , African Journals online Full-text available at http://www.ajbrui.com http://www.bioline.br/md http://www.ajol.com Received: Februaru 2007 Accepted (Revised): April 2007 Published May 2007 Short communication Cross Reactivities of Rabbit A...

OBJECTIVE Rheumatic fever (RF) and the antiphospholipid syndrome (APS) are autoimmune diseases that share similar cardiac and neurological pathologies. We assessed the presence of shared epitopes between M protein, N-acetyl-beta-D-glucosamine (GlcNAc) and beta2 glycoprotein-I (beta2GPI), the pathogenic molecules engaged in these autoimmune conditions. METHODS Sera from the APS patients were a...

Autoantibodies to components of the nucleolus are a unique serological feature of patients with scleroderma. There are autoantibodies of several specificities; one type produces a speckled pattern of nucleolar staining in immunofluorescence. In actinomycin D and 5,6-dichloro-beta-D-ribofuranosylbenzimidazole-treated Vero cells, staining was restricted to the fibrillar and not the granular regio...

Human heterophilic hemagglutinin to rabbit erythrocytes was quantitatively determined by the hemagglutination test and enzyme linked immunosorbent assay (ELISA) using rabbit erythrocyte pentaglycosyl ceramide (CPH), Gal (a 1-3) Gal (fl 1-4) GlcNAc (fl 1-3) Gal (fl 1-4) Glc-Cer as the antigen. The antibody levels in sera from 74 Hanganutziu-Deicher (H-D) antibody-positive patients were significa...

46% of sera from 30 children with rheumatic chorea showed IgG antibody reacting with neuronal cytoplasm of human caudate and subthalamic nuclei. The antibody was also detected in 14% of 50 children with active rheumatic carditis. 55 normal control sera, as well as 148 sera from a broad variety of other disease states showed a low prevalence (1.8-4.0%) of positive reactions. In rheumatic chorea ...

Objective: This study was aimed to access one of the hidden ANF in Indian bean and assessment its antioxidant, immunomodulatory, antiangiogenic, anti-cancer properties. Methods: beans were processed evaluated for presence through HA activity. Bean-ANF partially purified by ammonium sulfate precipitation (ASP) dialysis. Obtained Partially Purified bean-ANF (PPb-ANF) resolved on 12% SDS-PAGE, ana...

Background: In spite of available, and sensitive screening assay for detection of hepatitis B virus surface antigen (HBsAg), occasional cases of post-transfusion hepatitis B virus infection are still observed. The aim of the present study was to assess the prevalence of positive anti hepatitis B core (anti-HBc) and presence of HBV-DNA in serum sample of healthy blood donors negative for both HB...

BACKGROUND AND AIMS Presence of serum antibodies against Mycobacterium avium paratuberculosis (MAP) in Crohn's Disease (CD) as a disease characteristic remains controversial. In the present work, we assessed antibody reactivity of serum and intestinal fluid against four distinct MAP-antigens, including the recently identified MAP-specific lipopentapeptide (L5P). METHODS Immunoglobulin concent...

A radioimmunoassay for the binding protein for vitamin D and its metabolites (DBP) has been developed. Suitable rabbit anti-DBP antiserum was elicited after primary and one booster injection. Anti-DBP antisera, as well as antigroup-specific component antisera, produced a single, monospecific line of percipitation when reacted against purified DBP and human serum. DBP was iodinated with 125I and...