This study investigated the levels of the antibiotic tetracycline contained in cow milk, goat milk, butterfat, soft cheese and yoghurt samples. High performance liquid chromatography (HPLC) standard methods were used for the detection and quantification of tetracycline in 40 dairy products samples. All the analysed samples contained residues of tetracycline antibiotics. The mean tetracycline re...

Mastitis is known as the most common disease syndrome in the dairy industry. Treating mastitic lactating animals with antibiotics is a veterinary practice to cure the disease. However, the antibiotic used may persist in the milk for a period of time depending on drug selected, dosage applied, route administered, body weight of the animal treated, etc. Antibiotic residues in milk are of great co...

Isolation and Purification Megalomicin is an antibiotic mixture most readily isolated from the fermentation broth by adjusting the whole broth to a pH of 9.5 with dilute aqueous sodium hydroxide and extracting with approximately 2 volumes of ethyl acetate for each volume of broth. The solvent phase was separated and concentrated under vacuum to an oily residue. The biological activity of a 1:20...

The natural product thiopeptide antibiotic thiostrepton is shown to undergo facile epimerization at its thiazoline residue in favor of the naturally observed D-configuration, suggesting that a classical epimerase enzyme may not be involved in its biosynthesis.

The glycopeptide antibiotic vancomycin is unstable in solution. It undergoes a rearrangement involving the conversion of an asparagine residue to isoaspartate to give an antibiotically inactive species, CDP-I. Peptide analogs of bacterial peptidoglycan, such as Ac-DAla-D-Ala and di-Ac-L-Lys-D-Ala-D-Ala bind to vancomycin and stabilize the antibiotic against degradation and consequent loss of ac...

As a novel strategy to remove β-lactam antibiotic residues from fish tissues, utilization of β-lactamase, enzyme that normally degrades β-lactam structure-containing drugs, was explored. The enzyme (TEM-52) selectively degraded β-lactam antibiotics but was completely inactive against tetracycline-, quinolone-, macrolide-, or aminoglycosidestructured antibacterials. After simultaneous administra...

The practice of antimicrobial usage in African catfish (Clarias gariepenius) production and the presence of antimicrobial residue in catfish in Oyo State were investigated by cross sectional study. Twenty commercial fish farmers were interviewed using interviewers administered semi-structured questionnaire on the pattern of antibiotic usage, knowledge of occurrence and risks of antimicrobial re...