Antibody affinity is an important determinant of all antibody-antigen reactions. A new computer program, AFCRV, was developed to calculate binding constants with data from a radioimmunoassay on most microcomputers in the laboratory by using constant-ratio dilution curves. Evaluation of a homogeneous or heterogeneous antibody in the presence of a single antigen can be accomplished.

Antibody technologies are the most representative success-case in biopharmaceutical industry. Widely available purification fail providing a dedicated universal platform that can accommodate antibodies structural diversity, namely from non-human sources, as chicken IgY, and antigen-binding fragments. In this work, we took inspiration natural engineered antibody-binding ligands, to rationally de...

Abstract The red blood cell (RBC) antigen Wr a is low-prevalence first described in 1953 by Holman and assigned to the Diego system 1995. Because of its low prevalence, usually absent on commercial screening RBCs antibody identification panels. When Wr(a+) are available, corresponding antibody, anti-Wr , often found sera from healthy individuals, patients, pregnant women. Anti-Wr can cause both...

Two homologous lightly coupled dinitrophenyl conjugates of poly-L-lysine of differing average molecular sizes were compared with regard to their abilities to elicit in guinea pigs specific delayed hypersensitivity skin reactions, passive cutaneous anaphylaxis, and active Arthus reactions. Equal concentrations by weight (but not equimolar concentrations) of the two conjugates elicited equally in...

Most natural and synthetic antibodies are ‘unseen’. That is, the demonstration of their neutralization effects with any antigen requires laborious costly wet-lab experiments. The existing methods that learn antibody representations from known antibody–antigen interactions unsuitable for unseen owing to absence interaction instances. DeepAAI method proposed herein learns by constructing two adap...

Two monoclonal mouse antibodies with specificities for group B streptococcal capsular antigens were evaluated in assays for the identification of group B streptococci (GBS). One of these antibodies (A9) was shown to precipitate group B carbohydrate antigen in reactions with both purified group B antigen and antigen present in autoclave or enzyme extracts of GBS. A9 antibody was also specific fo...

The assumption that, at the equivalence point in specific precipitin reactions, the antigen molecule is completely covered with a single layer of antibody-globulin molecules has been shown to account fairly well for the antibody-antigen ratios of some representative native single proteins, and the pneumococcus S III hapten.

A murine IgM monoclonal antibody (MA-2C6) with κ-light chains directed against an antigenic determinant of outer surface protein A (OspA) of the Lyme disease spirochete, Borreliaburgdorferi, is produced. This antibody could bind specifically to OspA antigen of several isolates of B. burgdorferi, but not to the non-Lyme disease bacteria such as T. pallidum and B. hermsii. Antibody MA-2C6 was pur...