the stromal-vascular fraction separated from the adipocytes by centrifugation. T h e stromal-vascular cells were seeded ( 2 0 0 0 0 0 cells/ml; 2 m1/25 cm? tissue culture flask) in Medium 199 supplemented with 200h (v/v) fetal calf serum and incubated at 37°C. T h e adipocyte fraction from the digests was also collected and washed before being added ( I ml of cells/25 cm? tissue culture flask) ...

The non-histone nuclear phosphoprotein B2 (Mr 68,000; pI 6.5-8.2) was found to bind specifically defined fragments of DNA. With the use of monoclonal IgG2A antibodies prepared against this nuclear antigen, nucleosomal DNA fragments associated with phosphoprotein B2 were isolated and cloned. Nine cloned fragments were sequenced and analysed for similarity. The clone having the most similarity to...

The affinity of the origin-binding domain (OBD) of simian virus 40 large T antigen for its cognate origin was measured at equilibrium using a DNA binding assay based on fluorescence anisotropy. At a near-physiological concentration of salt, the affinities of the OBD for site II and the core origin were 31 and 50 nM, respectively. Binding to any of the four 5'-GAGGC-3' binding sites in site II w...

The roles of mutational and recombinational processes in the diversification of the exon encoding the antigen binding site in the murine major histocompatibility complex class II gene Ab were assessed by phylogenetic analysis of allelic nucleotide sequences. A total of 46 alleles of Ab exon 2 from 12 Mus species or subspecies and 2 Rattus species were sequenced after amplification by the polyme...

background: the development and progression of human tumors is accompanied by various cellular biochemical and genetic alterations. these events include tumor cells interaction with extracellular matrix molecules including hyaluronan and hyaluronan binding protein (ha-habp). hyaluronan is a large polysaccharide associated with pericellular matrix of proliferating, migrating cells, regulation of...

To map the DNA-binding domain of polyomavirus large T antigen, we constructed a set of plasmids coding for unidirectional carboxy- or amino-terminal deletion mutations in the large T antigen. Analysis of origin-specific DNA binding by mutant proteins expressed in Cos-1 cells revealed that the C-terminal boundary of the DNA-binding domain is at or near Glu-398. Fusion proteins of large T antigen...

Conventional serum antibody titer, which expresses level, does not provide antigen binding avidity of the variable region antibody, is essential for defense response to infection. Here, we quantified anti-SARS-CoV-2 receptor-binding domain (RBD) by competitive binding-inhibition activity (IC50) between SARS-CoV-2 S1 immobilized on DCP microarray and various RBD doses added expressed as 1/IC50 n...