A targeted heptasaccharide library was synthesised to prepare a heparan sulphate (HS) microarray. The array was probed with two glycan-binding proteins, HS 3-O-sulphotransferase 1 and antithrombin, demonstrating the binding selectivity between HS and proteins. The HS microarray technique will accelerate the understanding of the structure and function relationships of HS.

Context: Activation of the coagulation system and depletion of endogenous anticoagulants are frequently found in patients with severe sepsis and septic shock. Diffuse microthrombus formation may induce organ dysfunction and lead to excess mortality in septic shock. Antithrombin III may provide protection from multiorgan failure and improve survival in severely ill patients. Objective: To determ...

A difference in recognition of the adhesive glycoprotein vitronectin (also called S-protein, serum spreading factor, and epibolin) by monoclonal antibody 8E6 (Hayman EG, et al, Proc Natl Acad Sci USA 80:4003, 1983) was investigated using a competitive enzyme-immunosorbent assay and immunoaffinity chromatography. Recognition of vitronectin in serum was approximately 50-fold greater than recognit...

Finback-whale (Balaenoptera physalus L.) heparin was partially digested with a purified heparinase and an octasaccharide with high affinity for antithrombin III was isolated from the digest by gel filtration, followed by affinity chromatography on a column of antithrombin III immobilized on Sepharose 4B. This octasaccharide possessed high inhibitory activity for Factor Xa in the presence of ant...

Thrombin has attracted increasing attention as a possible mitogen for vascular smooth muscle cells in lesion development both after vascular injury and in atherogenesis. In this study, the ability of antithrombin III to inhibit alpha-thrombin-induced DNA synthesis and cell proliferation in human arterial smooth muscle cells was analyzed. We demonstrate a concentration-dependent initiation of DN...

The distribution of heparin between thrombin and antithrombin III in solutions containing both proteins has been calculated using a heparin-thrombin dissociation constant value, KgssMT, of 1.5 X lo-’ M and a heparin-antithrombin III dissociation constant value, KEgsMAT, of 2.0 x lo-’ M. The enhancing effect of heparin on the antithrombin III/thrombin reaction rate appeared to correlate with the...

High affinity heparin obtained by chromatography of clinical grade heparin on antithrombin 111-Sepharose was covalently coupled to antithrombin III using a three-step procedure: 1) introduction of free amino groups in the heparin molecule; 2) reaction of these amino groups with the bifunctional reagent tolylene2,4-diisothiocyanate; and 3) reaction of the remaining isothiocyanate group with amin...

The distribution of heparin between thrombin and antithrombin III in solutions containing both proteins has been calculated using a heparin-thrombin dissociation constant value, KgssMT, of 1.5 X lo-’ M and a heparin-antithrombin III dissociation constant value, KEgsMAT, of 2.0 x lo-’ M. The enhancing effect of heparin on the antithrombin III/thrombin reaction rate appeared to correlate with the...

The kinetics of inhibition of human thrombin and Factor Xa by antithrombin III or heparin cofactor II were examined under pseudo-first-order conditions as a function of the concentration of naturally occurring oversulphated chondroitin and dermatan sulphates. The sulphated glycosaminoglycans (GAGs) studied were chondroitin sulphate D (CSD) (GlcA-2-SO4-GalNAc-6-SO4), chondroitin sulphate K (CSK)...