The tomato (Lycopersicon esculentum) acid phosphatase-1 (Apase-1(1), EC 3.1.3.2) isozyme variant, genetically linked to the root-knot nematode resistance locus (Mi) on chromosome 6, has been purified by a rapid procedure from tomato cell suspension cultures. Peptide fragments of the purified enzyme were generated from trypsin and Lys-C endoprotease digests and separated by reverse-phase high-pe...

Phosphorus (P), in the form of phosphate ion (Pi), is a vital element contributing in biomolecule structures, metabolic reactions, signaling pathways and energy transfer within the living cells. The objective of the present study was to assess the influence of fungal infection on Pi metabolism in compare to the effects of phosphate stress in Arabidopsis. Quantification of total P contents showe...

Phosphorus (P), in the form of phosphate ion (Pi), is a vital element contributing in biomolecule structures, metabolic reactions, signaling pathways and energy transfer within the living cells. The objective of the present study was to assess the influence of fungal infection on Pi metabolism in compare to the effects of phosphate stress in Arabidopsis. Quantification of total P contents showe...

Phosphorus (P), in the form of phosphate ion (Pi), is a vital element contributing in biomolecule structures, metabolic reactions, signaling pathways and energy transfer within the living cells. The objective of the present study was to assess the influence of fungal infection on Pi metabolism in compare to the effects of phosphate stress in Arabidopsis. Quantification of total P contents showe...

H2PO4 (Pi) uptake rates, and root surface acid phosphatase (APase) rates were assessed in pitch pine (Pinus rigida Mill.) seedlings inoculated with the ectomycorrhizal fungi Laccaria bicolor (Maire) Pat., Paxillus involutus (Batsch.) Fr., or Pisolithus tinctorius (Pers.) Coker and Couch, and grown at 10 or 100 μM Pi in sand culture. Following a 6-week period of acclimation to the Pi regimes, se...

Serum acid phosphatase (AcPase) was measured by a colorimetric method utilizing adenosine 3' -monophosphate as substrate in 389 patients. In about half the cases blood was taken shortly after a rectal examination. The upper reference limit (mean + 2SD) for 116 cases with miscellaneous illness after eliminating outliers was 4.1 International Units per litre (U/I) at 37 degrees C, and no correlat...

S ince 1958 when Alfred C. Redfield (1) recognized the similarity between the ratios of elements in living biomass and those dissolved in the surrounding seawater, we have understood that microorganisms largely control the concentrations, distribution, and molecular makeup of nutritional resources in the ocean. The primary elemental ingredients for life, carbon (C), nitrogen (N), and phosphorus...

phosphorus (p), in the form of phosphate ion (pi), is a vital element contributing in biomolecule structures, metabolic reactions, signaling pathways and energy transfer within the living cells. the objective of the present study was to assess the influence of fungal infection on pi metabolism in compare to the effects of phosphate stress in arabidopsis. quantification of total p contents showe...

phosphorus (p), in the form of phosphate ion (pi), is a vital element contributing in biomolecule structures, metabolic reactions, signaling pathways and energy transfer within the living cells. the objective of the present study was to assess the influence of fungal infection on pi metabolism in compare to the effects of phosphate stress in arabidopsis. quantification of total p contents showe...

Assays on cell-free homogenates of Acanthamoeba castellanii reveal that the three hydrolases, acid phosphatase (APase), acid deoxyribonuclease, and acid ribonuclease (RNase), possess pH optima of 5.0, 4.8, and 5.2, respectively. These enzymes exhibit an enhanced sedimentation at 20,000 x g when sucrose is in the homogenizing buffer. Treatment of homogenates with Triton X-100 increases total enz...