Objective: The objective of the present study was to develop and validate a novel stability indicating reverse phase-high performance liquid chromatography (RP-HPLC) method for determination of β-acetyldigoxin, an active pharmaceutical ingredient (API). Methods: The chromatographic separation was carried out on Agilent Technologies 1200 series HPLC system equipped with photo diode array detecto...

Alpha-1-proteinase inhibitor (API) is one of the acute phase proteins. Following an inflammatory stimuli the concentration of API increased up to four folds. Accompanying these quantitative changes, there is qualitative alterations in the structure of carbohydrate moiety (glycosylation). To determine the alterations in the glycosylation of API in inflammation, API was isolated from the sera of...

This article focuses on stability indicating RP-HPLC method for simultaneous estimation of Frusemide and Amiloride Hydrochloride as API and in tablet dosage form, validation of developed method and its application in pharmaceutical companies. Acetonitrile, potassium di hydrogen phosphate and phosphoric acid were used. Chromatographic conditions comprised of C18 column (250 × 4.6 mm, 5 μm), mobi...

Syntax Notation One . . . . . . . 19 ACKbit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94 Activebit(S,G,Oif) . . . . . . . 38, 82, 90, 93 Address Resolution Protocol . . . . . . . . . . 7 Address Resource Record . . . . . . . . . . . . . 9 Adresse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 kodierte . . . . . . . . . . . . . . . . . . . . . . . . . 40 Adresspr...

Urine and serum samples collected from four standard-bred mares after and oral regimen administration of methocarbamol were extracted and analyzed. The method consisted of enzyme hydrolysis followed by a one-step liquid-liquid extraction, separation on a reversed-phase (RP-18) column, and detection using an ultraviolet (UV) detector. The confirmation was carried out using a liquid chromatograph...

The title octa-deca-trienoic acid derivative, C(18)H(26)O(4), was isolated from Silene maritima With. (Caryophyllaceae), the first time this natural compound has been found in the Caryophyllales order. This fatty acid has an 18-carbon backbone with three double bonds on trans (E) conformation and two carbonyl. In the crystal, molecules are linked via pairs of O-H⋯O hydrogen bonds, forming inver...

Activation of GPER exerts a protective effect in hypertension and ischemia-reperfusion models and relaxes arteries in vitro. However, our understanding of the mechanisms of GPER-mediated vascular regulation is far from complete. In the current study, we tested the hypothesis that GPER-induced relaxation of porcine coronary arteries is mediated via cAMP/PKA signaling. Our findings revealed that ...