Murine lipoproteins were separated into nine subfractions by a density gradient ultracentrifugal procedure. They were characterized by electrophoretic, immunological, chemical, and morphological analyses, and their protein moieties were defined according to charge, molecular weight, and isoelectric point. HDL predominated (approximately 500 mg/dl serum), the mode of its distribution being situa...

Apolipoprotein C-I11 (apoC-111) is a major protein of very low density lipoprotein (VLDL). The apoC-I11 polypeptide contains a carbohydrate chain containing galactosamine, galactose, and sialic acid attached in 0-linkage to a threonine residue at position 74. We have cloned the apoC-I11 gene from a subject whose serum contained unusually high amounts of apoC-111 lacking the carbohydrate moiety ...

Previously, a mutant apolipoprotein (apo) E, apolipoprotein E7 (Glu 244 ➝ Lys, Glu 245 ➝ Lys), has been identified in association with hyperlipidemia and atherosclerosis. To investigate the effects of its structural changes on lipoprotein metabolisms and its correlation with atherosclerosis, we characterized this mutant apoE with respect to its receptor-binding, heparin-binding, and lipoprotein...