The ability of various DNA damaging agents to induce asynchronous replication of polyoma DNA (APR) in rat cells carrying integrated copies of these DNA sequences may provide a useful model for understanding mechanisms of gene amplification. The present study has explored in detail the ability of UV irradiation to induce APR in the polyoma transformed rat fibroblast cell line H3. We have found t...

OBJECTIVES To assess the demographic, behavioural, psychosocial and structural factors associated with non-utilisation of HIV testing and counselling (HTC) services by female sex workers (FSWs) and men who have sex with men/transgender (MSM/TG). METHODS This study involved a cross-sectional design. We used the national surveillance survey data of 2012, which included 610 FSWs and 400 MSM/TG r...

Sulphate assimilation provides reduced sulphur for the synthesis of cysteine, methionine, and numerous other essential metabolites and secondary compounds. The key step in the pathway is the reduction of activated sulphate, adenosine 5'-phosphosulphate (APS), to sulphite catalysed by APS reductase (APR). In the present study, [(35)S]sulphur flux from external sulphate into glutathione (GSH) and...

The sulphate assimilation pathway provides reduced sulphur for the synthesis of the amino acids cysteine and methionine. These are the essential building blocks of proteins and further sources of reduced sulphur for the synthesis of coenzymes and various secondary compounds. Several recent reports identified the adenosine 5'-phosphosulphate reductase (APR) as the enzyme with the greatest contro...

Wright, Carola E., F. Haddad, A. X. Qin, P. W. Bodell, and K. M. Baldwin. In vivo regulation of b-MHC gene in rodent heart: role of T3 and evidence for an upstream enhancer. Am. J. Physiol. 276 (Cell Physiol. 45): C883–C891, 1999.—Cardiac b-myosin heavy chain (b-MHC) gene expression is mainly regulated through transcriptional processes. Although these results are based primarily on in vitro cel...

Anwar, M. A., M. Schwab, L. Poston, and P. W. Nathanielsz. Betamethasone-mediated vascular dysfunction and changes in hematological profile in the ovine fetus. Am. J. Physiol. 276 (Heart Circ. Physiol. 45): H1137–H1143, 1999.—Glucocorticoid administration to fetal sheep induces a sustained systemic blood pressure rise and an associated increase in femoral vascular resistance. We utilized a smal...

JIN KIM,1 WAN-YOUNG KIM,1 KI-HWAN HAN,1 MARK A. KNEPPER,2 SØREN NIELSEN,3 AND KIRSTEN M. MADSEN4 1Department of Anatomy, Catholic University Medical College, Seoul, Korea; 2Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-0951; 3Department of Cell Biology, Institute of Anatomy, University of Aarhu...

Campos, R. R., and R. M. McAllen. Cardiac inotropic, chronotropic, and dromotropic actions of subretrofacial neurons of cat RVLM. Am. J. Physiol. 276 (Regulatory Integrative Comp. Physiol. 45): R1102–R1111, 1999.—The cardiac actions of microinjecting sodium glutamate (0.5–2 nmol) among sympathetic premotor neurons of the subretrofacial nucleus in the rostral ventrolateral medulla (RVLM) were st...

Houpt, T. Richard, Hosook Yang-Preyer, Jessica Geyer, and Moria L. Norris. A rapid feedback signal is not always necessary for termination of a drinking bout. Am. J. Physiol. 276 (Regulatory Integrative Comp. Physiol. 45): R1156–R1163, 1999.—When a pig is deprived of drinking water, a deficit of body water develops that is corrected when the pig drinks to satiation. If food is available during ...

Challet, Etienne, Susan Losee-Olson, and Fred W. Turek. Reduced glucose availability attenuates circadian responses to light in mice. Am. J. Physiol. 276 (Regulatory Integrative Comp. Physiol. 45): R1063–R1070, 1999.—To test whether circadian responses to light are modulated by decreased glucose availability, we analyzed photic phase resetting of the circadian rhythm of locomotor activity in mi...