Aqueous Two Phase System (ATPS) or liquid-liquid extraction is used in biotechnology to recover valuable compounds from raw sources. In Two-Phase Systems, many factors influence the Partition coefficient, K, (which ratio of protein concentration top phase that bottom phase) and Recovery percentage (Rec%). this research, two systems ATPS were used: first, polyethylene glycol (PEG) 4000/Sodium ci...

Aqueous two-phase systems (ATPS) that are formed by mixing a polymer (usually polyethylene glycol, PEG) and a salt (e.g. phosphate, sulphate or citrate) or two polymers, and water can be effectively used for the separation and purification of proteins. The partitioning between both phases is dependent on the surface properties of the proteins and on the composition of the two phase system as ha...

The future challenges in bioprocessing include developing new downstream processes for the purification and manufacture of the protein based medicines of the future to relieve the predicted bottleneck being produced by increasingly high titres from fermentation processes. This review looks at the recent developments in centrifugal liquid-liquid partition chromatography using aqueous two-phase s...

Gigaporous poly(styrene-divinyl benzene) microspheres made via the surfactant reverse micelles swelling method had a controllable pore size of 100–500 nm. These microspheres had unique advantages in biomacromolecule separation and enzymes immobilization. However, the obtained microspheres adhered to each other in the preparation process. Though the weak aggregation could be re-dispersed easily ...

Aqueous two-phase systems (ATPS) based on ionic liquids (IL) and deep eutectic solvents (DES) are ecofriendly choices can be used to selectively separate compounds of interest, such as bioactive compounds. Bioactive nutrients nonnutrients animal, plant, microbial origin that benefit the human body in addition their classic nutritional properties. They also for technical purposes food active com...

Asymmetric cell division is common in biology and plays critical roles in differentiation and development. Unicellular organisms are often used as model systems for understanding the origins and consequences of asymmetry during cell division. Although basic as compared to mammalian cells, these are already quite complex. We report complete budding and asymmetric fission of very simple nonliving...

C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the...