ABSTRACT Armillaria mellea infects hundreds of plant species in natural and managed ecosystems throughout the Northern hemisphere. Previously reported nuclear genetic divergence between eastern and western U.S. isolates is consistent with the disjunct range of A. mellea in North America, which is restricted mainly to both coasts of the United States. We investigated patterns of population struc...

Armillaria mellea is a major plant pathogen. Yet, the strategies the organism uses to infect susceptible species, degrade lignocellulose and other plant material and protect itself against plant defences and its own glycodegradative arsenal are largely unknown. Here, we use a combination of gel and MS-based proteomics to profile A. mellea under conditions of oxidative stress and changes in grow...

Armillaria mellea is a serious pathogen of horticultural and agricultural systems in Europe and North America. The lack of a reliable in vitro fruiting system for heterothallic A. mellea has hindered research and required dependence on intermittently available wild-collected basidiospores of endemic genotypes, necessitating the use of variable genetic material in transformation studies. Here we...

We have purified an endo-exonuclease from the fruiting body of the basidiomycete fungus Armillaria mellea by using an ethanol fractionation step, followed by two rounds of column chromatography. The enzyme had an apparent molecular mass of 17500 Da and was shown to exist as a monomer by gel-filtration analysis. The nuclease was active on both double-stranded and single-stranded DNA but not on R...

Studying on Agaricales fungi collected from different parts of Mashhad and its suburbs (Khorasan province), 23 species were identificed. Among these, 18 species, namely, Lepiota cristata, Tricholoma sulphureum, Flammulina velutipes, Armillaria mellea, Pleurotus ostratus, Conocybe lactea, Agrocybe cylindracea, Mycena galopus, Psilocybe crobulus, Hypholoma   fasciculare, Agaricus bitorquis, Pholi...

The organic solvent extraction of Armillaria mellea showed different highly intrinsic fluorescent compounds either in rhizomorph or in hyphae (F , F , F , MF , MF and MF ). The characterization of ultraviolet and fluoro-spectrophotometry of these compounds has been exhibited. Thin layer chromatography and fluorescent spectrum of hyphae and rhizomorph indicated an additional compound (FH) ...

Wild growing mushrooms are a rich source of novel proteins with unique features. We have isolated and characterized trypsin inhibitors from two edible mushrooms, the honey fungus (Armillaria mellea) and the parasol mushroom (Macrolepiota procera), and from the poisonous death cap (Amanita phalloides). The trypsin inhibitors isolated: armespin, macrospin and amphaspin, have similar molecular mas...

Two prevalent issues with Gastrodia elata growing in a natural wild environment are the necessary symbiotic seed germination Mycena spp. and long cycle of protocorms into mature rhizomes Armillaria mellea. This study explored most suitable nutrients to germinate seeds developmental stages propagated protocorm/juvenile (jrhs) through tissue culture approach. Adding humic acid, mashed potato, pep...