We introduce a molecular toolbox for manipulation of neuronal gene expression in vivo. The toolbox includes promoters, ion channels, optogenetic tools, fluorescent proteins, and intronic artificial microRNAs. The components are easily assembled into adeno-associated virus (AAV) or lentivirus vectors using recombination cloning. We demonstrate assembly of toolbox components into lentivirus and A...

The functioning of living cells requires efficient and selective transport of materials into and out of the cell, and between different cellular compartments. Much of this transport occurs through nano-scale channels that do not require large scale molecular re-arrangements (such as transition from a 'closed' to an 'open' state) and do not require a direct input of metabolic energy during trans...

Recent experiments have revealed that the opening and closing of the channel is controlled by concertedly rotating and tilting the ends of the alpha-helices in the KcsA ion channels. We recognize these transmembrane alpha-helices as the generating lines of the ruled surface: the hyperboloid of one sheet. The twist-to-shrink feature of the hyperboloid is adopted by KcsA channels in gating the po...

A chute is characterized by a steep bed slope associated with torrential flow. This chute flow may be either smooth or stepped. The flow conditions in stepped channels are classified as nappe flow, transition flow and skimming flow. In this paper, hydraulic characteristics of flow regimes on the stepped channels are presented systematically under a wide range of discharge, channel slope and ste...

Microfluidic polymethylmethacrylate (PMMA) devices for study of particle transport in artificial porous media were designed and microfabricated using hot embossing with a brass mold insert containing a microchannel network with eight layers. After thermal bonding to enclose the microchannel network, a process protocol was applied to successfully remove bubbles in the PMMA device. Characterizati...

Aquaporins (AQPs) feature highly selective water transport through cell membranes, where the dipolar orientation of structured water wires spanning the AQP pore is of considerable importance for the selective translocation of water over ions. We recently discovered that water permeability through artificial water channels formed by stacked imidazole I-quartet superstructures increases when the ...

Mass transport at the sub-nanometre scale, including selective of gases, liquids and ions, plays a key role in systems such as catalysis, energy generation storage, chemical sensing molecular separation. Highly efficient biological channels living organisms have inspired design artificial with similar, or even higher, mass-transport efficiency, which can be used much larger scale. In this Revie...