Cultured porcine pulmonary artery endothelial monolayers actively transport albumin from interstitium to lumen. The active process favors interstitial to luminal transport by a factor of 10 even in the face of a 2:1 luminal:interstitial albumin gradient. The active process is abolished by treatment with 1 mM sodium cyanide. Dextran is not actively transported. This process could be important in...

The aim of the study was to evaluate Serum Ascites Albumin Gradient (SAAG) in ascitic dogs. Study was conducted at Referral Veterinary Poly Clinic, Indian Veterinary Research Institute, Izatnagar. Sick dogs were brought with clinical signs suggestive of distended abdomen and inappetance. General clinical examination, biochemical, ultrasound examination, abdominocentesis and peritoneal fluid exa...

The expression of albumin and alpha 1-inhibitor 3 genes was investigated in rat cell suspensions enriched in periportal (n = 10) and perivenous (n = 10) hepatocytes obtained by the digitonin-collagenase technique. The degree of enrichment of the cell suspensions was assessed: (1) by enzymic assays for the periportal marker alanine aminotransferase and for the perivenous marker glutamine synthet...

Although the mechanisms under which sex ratio is controlled in nature remain unknown, researchers have attempted to treat sperm in an effort to predetermine the sex of the offspring. The methods of sperm separation that have been repeatedly reported to isolate fractions rich in X- or Y-chromosome bearing sperm are serum albumin gradient swim-up and flow cytometry. However, the ability of the se...

The concentration of protein in the cerebrospinal fluid (CSF) is about 0.5 per cent that of plasma. This difference in concentration is generally considered (1) to be dependent upon the blood-CSF barrier. The normal concentration gradient of protein from a low level in the ventricles, 6 to 15 mg. per cent, to an intermediate level in the cisterna magna, 15 to 25 mg. per cent, to the highest lev...