p53 is the most commonly mutated gene in human cancers. Approximately 90% of the p53 gene mutations are localized between domains encoding exons 5 to 8. Sequencing methods currently available are tedious and time-consuming and are not suitable for routine laboratory testing. In an effort to identify a simple and rapid sequencing method, we analyzed 16 preselected breast tumors and 18 preselecte...

An expert system for amino acid sequence identification has been developed. The algorithm uses heuristic rules developed by human experts in protein sequencing. The system is applied to the chromatographic data of phenylthiohydantoin-amino acids acquired from an automated sequencer. The peak intensities in the current cycle are compared with those in the previous cycle, while the calibration an...

The paper presents a novel kind of sequencer hardware, called data sequencer. For many signal processing, multimedia, and other high-performance applications the new sequencer drastically reduces processor-to-memory bandwidth requirements, compared to conventional instruction sequencers. The data sequencer coordinates the parallel operation of multiple address generators, each providing indepen...

Source/Description: A phage library was constructed in dephosphorylated BamHI digested M13 from size-selected (220±1000 bp) turkey genomic DNA restricted with MboI. The library was screened with P-labeled (CA)16 and (GT)16 probes. Positive clones were sequenced with an automated DNA sequencer (ABI 373) at the Advanced Genetic Analysis Center, College of Veterinary Medicine, University of Minnes...

The complete amino acid sequence of versutoxin, a lethal neurotoxic polypeptide isolated from the venom of male and female funnel-web spiders of the species Atrax versutus, was determined. Sequencing was performed in a gas-phase protein sequencer by automated Edman degradation of the S-carboxymethylated toxin and fragments of it produced by reaction with CNBr. Versutoxin consisted of a single c...

AIM Rapid detection of H.pylori strains by PCR-Sequencing. METHODS 16S rDNA amplification by PCR from template genomic DNA, confirmation of amplicon size by agarose gel electrophoresis, sequencing of amplicons by automated sequencer, analysis of sequences by NCBI -BLAST software. RESULTS The PCR -Sequencing and analysis of the sequence data by BLAST resulted in detection of the strain to be...

Ù This work was supported by Sandia Corporation under Contract No. DE-AC04-94-AL85000 with the U.S. Department of Energy. Abstract A CORBA-based distributed object software system was developed for Sandia’s Agile Manufacturing Testbed (SAMT). This information architecture supports the goals of agile manufacturing: rapid response to changing requirements; reduction in both time and cost of the p...