alpha-Cyclodextrin specifically inhibited raw starch digestion by Bacillus subtilis 65 alpha-amylase. The raw starch digestibility and alpha-cyclodextrin-Sepharose 6B adsorbability of this alpha-amylase were simultaneously lost when the specific domain corresponding to the affinity site essential for raw starch digestion was deleted by proteolysis. Occurrence of the affinity site on raw-starch-...

AmyL, an extracellular alpha-amylase from Bacillus licheniformis, is resistant to extracellular proteases secreted by Bacillus subtilis during growth. Nevertheless, when AmyL is produced and secreted by B. subtilis, it is subject to considerable cell-associated proteolysis. Cell-wall-bound proteins CWBP52 and CWBP23 are the processed products of the B. subtilis wprA gene. Although no activity h...

BACKGROUND The microbial cell factory Bacillus subtilis is a popular industrial platform for high-level production of secreted technical enzymes. Nonetheless, the effective secretion of particular heterologous enzymes remains challenging. Over the past decades various studies have tackled this problem, and major improvements were achieved by optimizing signal peptides or removing proteases invo...

The current study optimized the production of α-amylase by Bacillus subtilis using solid state fermentation (SSF) process. The agricultural by-product of rice straw was utilized as a substrate support for the fermentation process. The characterization of untreated and NaOH-treated rice straw was conducted using Scanning Electron Microscope (SEM) and Energy Dispersive X-ray Analysis (EDX). The o...

BACKGROUND The maltogenic amylase from Bacillus stearothermophilus (BSMA) is a valuable biocatalyst that has been used to transglycosylate natural glycosides to improve solubility. To ensure safety, BSMA was produced in Bacillus subtilis, using new shuttle vector-based expression vectors. The transglycosylation of puerarin was also conducted with crude BSMA and analyzed. RESULTS Two expressio...

BACKGROUND Bacillus subtilis is widely used as a cell factory for numerous heterologous proteins of commercial value and medical interest. To explore the possibility of further enhancing the secretion potential of this model bacterium, a library of engineered strains with modified cell surface components was constructed, and the corresponding influences on protein secretion were investigated by...

The maltose α-amylase AmyM from Bacillus stearothermophilus can be used for flour modification, baked goods preservation, and production. Here, we optimized the recombinant expression of in subtilis WB800 via several strategies. By screening optimal promoter, a double promoter combination (P43 PamyL) could improve level by 61.25%, compared with strong P43. Then, secretion efficiency over-expres...

• First time HPTLC- Fusarium avenaceum bioassay using mycelium suspension HPTLC-EDA combined with HPTLC-DART-HRMS and HPTLC-ESI-HRMS Preparative-scale isolation of eight multipotent clerodane diterpenes cholinesterase, glucosidase, amylase microbial inhibitory potential compounds Giant goldenrod ( Solidago gigantea Ait.) root extract was screened for bioactive by high-performance thin-layer chr...