Utilization of naturally-occurring dissolved free and combined amino acids (DFAA and DCAA) and dissolved DNA (D-DNA) was studied in batch cultures of bacteria from 2 shallow marine environments, Santa Rosa Sound (SRS), Florida. USA, and Flax Pond (FP), Long Island, New York, USA. In addition to control experiments, cultures were enriched with various sources of C and N. In the SRS cultures, net...

Well-differentiated cultures established from airway epithelia of patients with cystic fibrosis (CF cultures) exhibited goblet cell hyperplasia, increased secretion of mucus, and higher basal levels of interleukin-8 than similarly cultured cells from healthy donors. Upon apical infection with low doses (10(4) to 10(5) CFU) of Burkholderia cenocepacia isolate BC7, the two cultures gave different...

results among the 4,506 studied patients with urine cultures, 300 (6.65%) and 4,206 (93.35%) urine samples showed positive and negative bacterial growth, respectively. the rates of urine cultures with bacterial growth for women and men were 90.66% and 9.44%, respectively. with regard to the bacterial growth, 90.33% were gram-negative and 9.67% were gram-positive. the most common isolated agent ...

Microorganisms are able to form biofilms within respiratory secretions. Methods to disaggregate such biofilms before utilizing standard, rapid, or high throughput diagnostic technologies may aid in pathogen detection during ventilator associated pneumonia (VAP) diagnosis. Our aim was to determine if sonication of endotracheal aspirates (ETA) would increase the sensitivity of qualitative, semi-q...

Gymnodinium catenatum Graham (Dinophyceae) is one of several marine dinoflagellates responsible for outbreaks of paralytic shellfish poisoning (PSP), a problem that is considered to be increasing globally. Bacteria associated with these dinoflagellates have been implicated as potentially involved with the production of PSP toxins, and this study sought to identify whether there was a link betwe...