background: vp4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. vp4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. methods: simian rotavirus sa11 gene 4 cdna was cloned into a cloning plasmid pdonrtm by recombination reaction using clonase ii enzyme mix. the resulting clone was c...

Abstract Background Biological pesticides, especially baculovirus, often lose their activity under the influence of external light, temperature, and other changes. This limited application them. The present study was aimed to prolong biological ensure efficacy a pesticide using microencapsulation technology. Results In this study, gelatin/carboxymethylcellulose (CMC)- Spodoptera litura nucleopo...

conclusions: the recombinant baculovirus constructed in this work has proper characteristics to produce h5n1 influenza virus-like particles in sf9 cells. results: restriction map of pfastbacihnm1 plasmid confirmed the fidelity of the clone. the pcr carried out on the recombinant bacmids as template indicated that a proper homologous recombination has occurred between pfastbacihnm1 donor plasmid...

The baculovirus is a classic example of a parasite that alters the behavior or physiology of its host so that progeny transmission is maximized. Baculoviruses do this by inducing enhanced locomotory activity (ELA) that causes the host caterpillars to climb to the upper foliage of plants. We previously reported that this behavior is not induced in silkworms that are infected with a mutant baculo...

A bicistronic baculovirus expression vector and fluorescent protein-based assays were used to identify the sequences that possess internal translation activity in baculovirus-infected insect cells. We demonstrated that the 5' untranslated region (5'UTR; 473 nucleotides) of Perina nuda virus (PnV) and the 5'UTR (579 nucleotides) of Rhopalosiphum padi virus (RhPV), but not the IRES sequence of Cr...

Spodoptera exigua microarray was used to determine genes differentially expressed in S. exigua cells challenged with the species-specific baculovirus SeMNPV as well as with a generalist baculovirus, AcMNPV. Microarray results revealed that, in contrast to the host transcriptional shut-off that is expected during baculovirus infection, S. exigua cells showed a balanced number of up- and down-reg...

Insect baculoviruses are capable of infecting mammalian glial cells in the central nervous system. We investigated in the current study the feasibility of using the viruses as toxin gene vectors to eliminate malignant glioma cells in the brain. We first confirmed that glioma cells were permissive to baculovirus infection, with variable transduction efficiencies at 100 viral particles per cell a...

Although microarray and expressed sequence tag (EST)-based approaches have been used to profile gene expression during baculovirus infection, the response of host genes to baculovirus infection and the interaction between baculovirus and its host remain largely unknown. To determine the host response to Bombyx mori nucleopolyhedrovirus infection and the dynamic interaction between the virus and...

Baculoviruses have evolved to control the defense mechanism of host cells and to replicate in the nuclei of infected cells efficiently. However, little is known about the dynamics and mechanism of baculovirus replication. To elucidate this, we have investigated the function of baculovirus genes and the baculovirus-host interaction using Bombyx mori nucleopolyhedrovirus (BmNPV). Here, we describ...