objective(s): adeno-associated virus type 2 (aav2) vectors are widely used for both experimental and clinical gene therapy. a recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. in this study, a fast and simple method is presented to generate aav2 vector helper plasmids encodin...

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as a generalization of the triple i method, the universal triple imethod is investigated from the viewpoints of both fuzzy reasoningand fuzzy controller. the universal triple i principle is putforward, which improves the previous triple i principle. then,unified form of universal triple i method is established based onthe (0,1)-implication or r-implication. moreover, the reversibilityproperty o...

  Objective(s): Adeno-associated virus type 2 (AAV2) vectors are widely used for both experimental and clinical gene therapy. A recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. In this study, a fast and simple method is presented to generate AAV2 vector helper plasmids encod...

Over the last 20 years, we have seen dramatic business and academic interest in building sustainable organizations. Sustainable organizations have the capacity to endure and simultaneously satisfy a triple bottom line of economic, environmental and human performance. Yet, in comparison to the environmental and economic dimensions of sustainability, substantially less attention has been focused ...

The performance of triple stores is one of the major obstacles for the deployment of semantic technologies in many usage scenarios. In particular Semantic Web applications, which use triple stores as persistence backends, trade performance in for the advantage of flexibility with regard to information structuring. In order to get closer to the performance of relational database-backed Web appli...

-1 Programmed ribosomal frameshifting (PRF) in synthesizing the gag-pro precursor polyprotein of Simian retrovirus type-1 (SRV-1) is stimulated by a classical H-type pseudoknot which forms an extended triple helix involving base-base and base-sugar interactions between loop and stem nucleotides. Recently, we showed that mutation of bases involved in triple helix formation affected frameshifting...