Plasmid pGNB1 was isolated from bacteria residing in the activated sludge compartment of a wastewater treatment plant by using a transformation-based approach. This 60-kb plasmid confers resistance to the triphenylmethane dye crystal violet and enables its host bacterium to decolorize crystal violet. Partial sequencing of pGNB1 revealed that its backbone is very similar to that of previously se...

Ultraviolet (UV) reception is useful for such basic behaviors as mate choice, foraging, predator avoidance, communication, and navigation, whereas violet reception improves visual resolution and subtle contrast detection. UV and violet reception are mediated by the short wavelength-sensitive (SWS1) pigments that absorb light maximally (λmax) at ~360 nm and ~395 to 440 nm, respectively. Because ...

Photolysis and photocatalysis of Lauth's violet using nano-titanium dioxide at various aqueous buffer and non-buffer pHs. The effects of some various operating parameters such as initial dye concentration, medium pH, illumination time and catalyst amount have been investigated in a photoreactor equipped with high pressure mercury lamp. Spectrophotometric methods supported that the dye is al...

Carboxymethyl cellulose (CMC)/titanium dioxide (TiO2) was prepared using gamma irradiation at different doses. used as a matrix and TiO2 in contents added (0.25, 0.5, 1, 1.5 wt%) filler. The polymer composite films were irradiated doses of 5, 10 15 kGy 60Co γ-ray to form crosslinked network structure. described by diagnostic procedures including X-ray diffractometer, scanning electron microscop...

In this study, 12 varieties of statice (Limonium sinuatum L.) were collected from famous flower growing areas (Van Thanh, Thai Phien, Da Thien, Ha Dong and Trai Mat) in Lam Dong. Young foliage flowering cultivars after 45 days planting at the experimental site was for DNA extraction genetic correlation analysis using RAPD with 13 random primers. Results showed that out 145 bands, there 133 poly...

A new type of fluorogenic substrates for proteases based on the leaving group cresyl violet has been synthesized. Cresyl violet is not fluorescent when amino acids or peptide groups are attached but becomes highly fluorescent after proteolytic liberation. Its fluorescence shows linearity with concentration and barely any fading. The properties of Ala-Pro-cresyl violet as substrate for dipeptidy...

Photoactivatable fluorescent proteins bring new dimension to the analysis of protein dynamics in the cell. Protein tagged with a photoactivatable label can be visualized and tracked in a spatially and temporally defined manner. Here, we describe a basic rational design strategy to develop monomeric photoactivatable proteins using site-specific mutagenesis of common monomeric red-shifted fluores...